miR-146aC〉G,miR-149T〉C基因多态性与缺血性脑卒中易感性的研究

目的：探讨中国汉族人群中miR-146aC〉G,miR-149T〉C基因多态性与缺血性脑卒中易感性的关系。方法：利用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）的方法检测196例缺血性脑卒中患者和205例健康对照中miR-146aC〉G,miR-149T〉C的基因型,统计学方法比较两组间基因型及等位基因分布差异。结果：miR-146aC〉G位点各基因型在病例组和对照组分布无明显差异,但等位基因G会增加缺血性脑卒中的患病风险;miR-149T〉C位点各基因型在病例组和对照组分布无明显差异。在分层分析中,miR-146aC〉G会增加女性和非高血压患者缺血性脑卒中的患病风险,miR-149T〉C会增加非高血压患者缺血性脑卒中的患病风险。结论：miR-146aG等位基因,miR-149C等位基因与汉族人群缺血性脑卒中易感性有一定的相关性。

Study on the Association of miR- 146aC〉G,miR- 149 T〉C Polymorphism with Susceptibility to Ischemic Stroke

Objective： To investigate the relationship between miR-146aC〉G, miR-149 T〉C and susceptibility to ischemic stroke in a Chinese population. Methods： Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） were used to determine the genotype of 196 ischemic stroke patients and 205 healthy controls. Statistical analyses were used to compare the distribution of the genotypes and alles between cases and controls. Results： There were no significant differences of the miR-146aC〉G, miR-149 T〉C genotype between the cases and controls, but alle G of miR-146a can increase the risk of ischemic stroke. In the stratified analyses, miR-146aCG＋GG showed a significantly increased risk of ischemic stroke compared with the CC genotype of the female and normotensive subgroups; miR-149TC＋CC was connected to ischemic stroke in the normotensive subgroup. Conclusion： miR-146a alle G, miR-149 alle C were associated with an increased risk ofischemic stroke in chinese.