共振喇曼光谱技术在恶性肿瘤诊断中的应用

目的：探讨共振喇曼光谱技术用于早期恶性肿瘤诊断的研究。方法：利用氩离子激光作为线偏振光的特点,采集偏振荧光光谱,对荧光光谱的偏振态进行分析。利用不同荧光物质的荧光可能具有不同偏振态的特点减少其它荧光物质的荧光对光谱分析的影响。血清样品产生的荧光也具有确定的偏振性。对所检测病人血清经激光分析仪进行喇曼光谱技术分析,光谱数据经计算机软件处理,自动显示图谱和数据,并直接给出各项指标及诊断提示。本结果与细胞病理学结果进行了对照研究。结果：恶性肿瘤样本176例,检测出阳性病例141例,阳性符合率为80.1%;良性肿瘤样本52例,4例阳性,假阳性率为7.7%;正常体检样本248例,检测结果均为阴性。结论：喇曼光谱技术适用于肿瘤初筛、普查及早期诊断,有推广应用前途。

Application of Ultraviolet Resonance Raman Spectroscopy in Diagnosis of Human Malignancies

Objective：To investigate the resonance Raman spectroscopy for early diagnosis of malignant tumors.Method：Using the characteristics of argon ion laser as linearly polarized light,polarized fluorescence spectra were collected,and the fluorescence polar-ization spectrum were analyzed.The features of different fluorescent substances having different polarization were utilized to reduce the impact of other fluorescent material on the spectrum.The serum samples also have authentic fluorescence polarization.Raman spec-troscopy detection of serum was undertaken by laser analyzer.The spectral data were processed by application software to show data and display spectra patterns.The indicators and diagnostic tips were automatically given.Results：In 176 malignant tumor samples,141 cases showed positive data,the positive consistent rate was 80.1%.In 52 benign tumor samples,4 cases were positive,the false positive rate was 7.7%.The 248 normal samples were uniformly negative.Conclusion：Raman spectroscopy was a promising application for cancer screening and early diagnosis.