周期性机械应力对髓核细胞增殖和细胞外基质表达的影响

目的：探讨周期性机械应力对髓核细胞增殖和细胞外基质表达的影响。方法：对兔髓核细胞进行体外细胞培养,对细胞施加周期性机械应力（0.25Mpa,0.1Hz）。实验分为2组,不加压组和加压组,不加压组置于单纯旋转式生物反应器内,加压组每天置于周期性机械应力场内2小时。分别于3天,7天检测细胞数目以及聚集蛋白聚糖（aggrecan）和Ⅱ型胶原的基因表达。结果：髓核细胞的增殖和聚集蛋白聚糖、Ⅱ型胶原基因的表达水平与周期性压力密切相关,在周期性机械应力刺激下髓核细胞增殖明显,细胞外基质的分泌增加,组织工程髓核细胞的活性显著提高。结论：周期性机械应力能够显著促进髓核细胞增殖,同时上调聚集蛋白聚糖、Ⅱ型胶原的基因的表达。

Cell Proliferation and Extracellular Matrix Expression in Nucleus Pulposus Cells under Cyclic Hyolrostatic Pressure

Objective：To investigate the effect of cyclic hyolrostatic pressure on cell proliferation and extracellular matrix expres-sion in the Nucleus pulposus cells.Methods：The Nucleus pulposus cells of rabbits were cultured in vitro,and the cells underwent cyclic hydrostatic loading（0.25Mpa,0.1Hz）.Cells were divided into 2 groups,no pressure groups and pressure groups.the cells of no pressure groups were placed in a simple rotating bioreactor,while the cells of the pressure groups were placed on the cyclic mechanical stress 2 hours a day.The cell？s number and the gene expression of aggrecan and type Ⅱ collagen were detected at 3 days and 7 days.Results：The cell proliferation of nucleus pulposus and the expression of aggrecan and collagen type Ⅱ in nucleus pulposus was assocated with the cyclic hyolrostatic pressure.The cyclic hyolrostatic pressure of nucleus pulposus cells increased cell proliferation and secretion of extracellular matrix,and significantly increased the activity of nucleus pulposus cells of tissue engineering.Conclusions：The cyclic mechanical stress can significantly promote the cell proliferation of nucleus pulposus,and increased aggrecan and typeⅡcollagen gene expression.