下丘脑室旁核胃动素对消化功能影响的研究

目的：研究下丘脑室旁核注入胃动素及其拮抗剂对大鼠消化功能和体重增长的研究。方法：将剂量为0.005-5nmol的motilin和GM109注入大鼠下丘脑室旁核,1小时后可观察到大鼠摄食量显著增加并持续到两小时后。进食量的计算是通过预先称量好的鼠粮和应用药物20分钟、1小时、两小时后剩余数量比较而得出。实验持续一周。将实验组和对照组的进食量和体重进行比较。结果：室旁核注入胃动素5nmol的实验组和合并应用GM1090.005nmol的实验组在应用药物后1小时和2小时,可观察到摄食量显著增加（p〈0.01）,一周后体重也增加（p〉0.05）,然而摄食量的增加有显著性差异,体重的增加并无显著性差异。其他实验组也没有观察到显著性差异。结论：胃动素有调节消化运动,促进胃肠排空,促进食欲的作用。可能由于胃肠排空是频繁的,没有充裕的时间消化吸收,从而体重增加无显著性差异。

The Impact study of motilin digestive function in hypothalamus paraventricular nucleus

Objective：To investigate the effects of paraventricular hypothalamic nucleus（PVN）administration of motilin and motilin receptor antagonist GM-109 on ingestion and body weight gain in rats.Methods：Motilin and GM-109 were injected in PVN at doses of 0.005-5 nmol.A significant increasing ingestion was observed 1h after PVN injection motilin 3nmol/rat and continued for 2h.Ingestion was measured by decreasing of pellets between the preweighted pellets and the uneaten pellets at 20 min,1 and 2 h after drug.The mice were weighted.Daily PVN administration of motilin and GM-109,the weight of the rats were detected for 1week.Body weight,food intake were analyzed and compared with those of the rats of ACSF（NaCl,138.9 mmol;KCl,3.4 mmol;CaCl2,1.26 mmol;NaHCO3,4 mmol;NaH2PO4·2H2O,0.6 mmol;and glucose,5.6-mmol）-treated controls.Results：PVN administration of motilin 5nmol/rat increased significantly the food intake after 1 and 2 h.The body weight increased 7days later.Food intake increased significantly（p0.01）,howev-er,body weight gained slight（p0.05）.However,which was not observed in PVN administration GM-109 at doses of 0.005-5nmol/rat,or ACSF-injected controls.These effects indicate that motilin is involved in the regulation of digestive motility and that motilin receptors are present in the brain and may have a role in the regulation of food intake.Conclusion：motilin modulates digestive movement,and pro-motes gastric emptying and appetitive effect.May be due to gastric emptying is frequent,no plenty of time to digest absorb,thus weight gain no significant differences.