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紫花牡荆素诱导人肝癌HepG2细胞凋亡的研究
引用本文:舒宝莲,曾斌,廖爱军,张杰,丁由,石巍.紫花牡荆素诱导人肝癌HepG2细胞凋亡的研究[J].生物磁学,2009(20):3841-3844.
作者姓名:舒宝莲  曾斌  廖爱军  张杰  丁由  石巍
作者单位:南华大学附属一医院消化内科,湖南衡阳421001
基金项目:基金项目:湖南省自然科学基金(08JJ3032);湖南省卫生厅科研计划项目(B2007100)
摘    要:目的:研究紫花牡荆素(Casticin)对肝癌HepG2细胞增殖抑制和凋亡诱导的作用,并探讨其作用机制。方法:用终浓度为0、0.5、1.0、2.0umol/L的Casticin作用于HepG2细胞,于12、24、48h后采用MTT法检测细胞增殖抑制率;Hoechst33342核染色,观察细胞形态学变化;24h后收集各组肝癌HepG2细胞,流式细胞术检测细胞周期及凋亡率;RT-PCR检测survivin mRNA表达。结果:MTT法检测显示,Casticin对肝癌HepG2细胞有增殖抑制作用,并存在浓度和时间依赖关系;Hoechst33342染色后,可见核染色质凝集,凋亡细胞呈致密浓染,与对照组相比,Casticin处理后凋亡细胞比例增加;Casticin作用24h后,细胞被阻滞于G2/M期,随药物质量浓度的增加,细胞凋亡率逐渐增加;RT-PCR结果显示,Casticin下调肝癌HepG2细胞survivin mRNA表达。结论:Casticin在体外对肝癌HepG2细胞有明显的增殖抑制和凋亡诱导作用,初步推断Casticin诱发肝癌细胞凋亡与其对survivin基因表达的抑制有关。

关 键 词:紫花牡荆素  肝癌  凋亡  survivin

Study on apotosis of human hepatocellular carcinoma HepG2 cells induced by Casticin
Institution:SHU Bao-lian, ZENG Bin, LIAO Ai-jun, ZHANG Jic, DING You, SHI We (Department of Gastroenterology o f First Affiliated Hospital of Nanhua University, Heng Yang, 421001, China )
Abstract:Objective: To study the effects of Casticin on the proliferation and apoptosis induction of human hepatocellular carcinoma HepG2 cells, and explore the mechanism. Methods: Human hepatocellular carcinoma HepG2 cells were treated with 0, 0.5, 1.0, 2.0umol/1 Casticin and the cell proliferation inhibition rates were determined by MTT assay 12h,24 h and 48 h after treatment. Morphological changes of cell nuclei were observed by Hoechst 33342 staining. In addition, cell apoptosis rates and cell cycle were determined by flow cytometry, and RT-PCR was employed to detect the expression of survivin mRNA. Results: It was demonstrated by MTT assay that the growth of HepG2 cells was inhibited by Casticin in a time and dose-dependent manner. The chromatine concentration and apoptotic cells with high concentrations were observed by Hoechst 33342 staining. The percentage of cells in G2 / M phase increased ,After treatment with Casticin, apoptosis rate of HepG2 cells increased with the increase of concentration (P〈 0.05 ). It was indicated by RT-PCR that the expression of survivin mRNA in HepG2 cells treated with Casticin was downregulated. Conclusion: Casticin has inhibitory effects on HepG2 cell proliferation and can induce cell apoptosis in vitro, which may be related to the inhibition of the expression of survivin mRNA.
Keywords:Casticin  Hepatocellular carcinoma  Apoptosis  Survivin
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