DADS对人小细胞肺癌NCI-H446细胞系增殖抑制的研究

目的：研究二烯丙基二硫（diallyldisulfide，DADS）对人小细胞肺癌NCI．H446细胞增殖的抑制作用，并探讨其作用机制。方法：体外培养NCI-H446细胞，采用MTT、细胞计数实验方法检测DADS抑制NCI—H446细胞增殖；通过HE染色和AO—EB荧光染色方法，观察DADS处理后NCI—H446细胞的形态学改变。结果：MTT结果显示：DADS作用于NCI—H446细胞48h后，代谢MTT的能力明显降低，显示出较强的细胞毒性反应，IC50值介于20-40μg／ml之间。细胞计数结果表明：DADS作用于NCI—H446细胞后，随DADS浓度增加NCI—H446细胞倍增时间延长。HE染色显示：NCI—H446细胞经DADS处理24h后，与对照组相比，细胞体积变小，胞浆丰富，细胞核变小，染色变淡。AO-EB荧光染色显示：NCI-H446细胞经DADS处理24h后，与对照组相比，细胞皱缩、呈圆形，胞质黄色或橘红色，细胞核或细胞质内可见致密浓染的黄绿色或橘红色荧光，并可见橘红色碎片且随DADS浓度增加，随DADS浓度增加细胞密度逐渐减少。结论：DADS能抑制体外培养的NCI—H446细胞增殖，作用效果与药物浓度及作用时间相关。

Proliferation Inhibited in NCI-H440 Cells with DADS

Objective： To investigate the effect and the mechanism of DADS on the growth of human small cell lung cancer NCI-H446 cells in vitro. Methods： NCI-H446 cell lines were cultured in vitro. Cell proliferation inhibition was measured by MTT assay and cell count experiments. Cell morphologic change was observed by HE staining and AO-EB fluorescence staining. Result： MTT assay showed that the ability of the metabolic MTT reduced significantly and it had a strong cytotoxic response after cultured 48 hours by DADS. IC50 values located between 20 - 40 μg/ml. Cell count experiments showed that NCI-H446 cells average doubling time extended after exposure to different concentration DADS. HE staining showed that cell volumes and nucleus become smaller while cytoplasm became abundant and stain hard 24 hours after H446 cellular exposure to DADS. AO-EB fluorescence staining showed that the cells morphoous and colour had huge changed that dealed with DADS. Conclusion： DADS could markedly inhibit the proliferation of NCI-H446 and the effect depended on the concentration and the time of DADS.