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动物组织蛋白酶L=like基因家族的进化分析
引用本文:张耀洋,王博,姚蜜蜜,蔡中华.动物组织蛋白酶L=like基因家族的进化分析[J].生物磁学,2014(8):1401-1406,1427.
作者姓名:张耀洋  王博  姚蜜蜜  蔡中华
作者单位:[1]清华大学生命科学学院,北京100084 [2]清华大学深圳研究生院深圳海洋微生物资源筛选与利用公共服务平台,广东深圳510000
基金项目:国家重点基础研究发展计划“973”前期项目(2012CB426504);国家海洋局公益性项目(201205020-09;20130502)
摘    要:目的:组织蛋白酶L-like家族是在溶酶体中发现的一类非常重要的半胱氨酸组织蛋白酶。其主要功能为催化各种蛋白质的水解,并通过水解蛋白质参与到许多的生理调节过程当中。根据序列比对分析和传统的功能分类,在动物中,组织蛋白酶L.1ike家族成员包括组织蛋白酶L、V、S、K、H和F。但是这些家族成员之间的进化关系仍然没有详细研究分析清楚。本课题主要研究组织蛋白酶L-like家族成员之间的进化关系。方法:本研究通过搜集整理22个物种的177条组织蛋白酶L-1ike家族蛋白的序列,并构建系统发育进化树来分析组织蛋白酶L-like家族各成员之间的进化关系。结果:序列数据结果显示,串联重复在组织蛋白酶L-1ike家族的进化过程中发生。斑马鱼的组织蛋白酶L,爪蟾的组织蛋白酶S和K,大鼠和小鼠的组织蛋白酶L都发生过明显的串联重复事件。进化树结果显示了组织蛋白酶H、S和K、L和V之间的进化关系,组织蛋白酶S和K在脊椎动物出现的进化过程中,从组织蛋白酶L中分化出来,与他们在脊椎动物体内的特异性功能,以及脊椎动物在进化过程中分化产生的特异性功能相对应。结论:在物种进化的过程中,组织蛋白酶L-1ike家族成员F、H、S和K、L和V按时间顺序分化,这表明组织蛋白酶L-1ike基因家族结构和功能的分化与新的物种和新的功能出现密切相关。

关 键 词:组织蛋白酶  串联重复  系统进化

Phylogenetic Analysis of Cathepsin L-like family in Animal
ZHANG Yao-yang,WANG Bo;,YAO Mi-mi,CAI Zhong-hua.Phylogenetic Analysis of Cathepsin L-like family in Animal[J].Biomagnetism,2014(8):1401-1406,1427.
Authors:ZHANG Yao-yang  WANG Bo;  YAO Mi-mi  CAI Zhong-hua
Institution:1 Department of Biological Science and Biotechnology, Tsinghua University, Beijing, 100084, China; 2 Shenzhen Public Service Platforms of Marine Microbial Resource Screening and Exploitation, Graduate School of Tsinghua University, Shenzhen, Guangdong, 510000, China)
Abstract:Objective: The cathepsin L family is an important cysteine protease found in lysosome.Its main function is catalyzing proteolysis, and participating in numerous biological processes by proteolysis. The family contains cathepsin L, V, S, K, H and F in verte- brates according to sequence alignment and traditional fimction classification. The evolutionary relationship of this family members has not been clearly elucidated. Our study focused on the phylogeny of Cathepsin L-like family members. Methods: 177 cathepsin L-like gene and protein sequences of 22 species were sourced on database. Phylogenetic tree was constructed to trace the evolutionary relation- ship of the cathepsin L family genes with these sequences. Results: Tandem repeat duplication happened in the evolutionary history of cathepsin L family. Cathepsin L in zebrafish, cathepsin S and K in xenopus, cathepsin L in mouse and rat experienced obvious tandem re- peat event. The results of phylogeny trees showed the evolution relationship of cathepsin H, S and K, L and V. Cathepsin S and K differ- entiated from cathepsin L in the appearance of vertebrates, which coincide with their special fi.mction role in the differentiation of verte- brates. Conclusions: Cathepsin L-like family members F, H, S and K, L and V diverged chronologically in the evolution of species. This indicates that the structure and function divergence of cathepsin L-like family and the appearance of new species and new function are closely related.
Keywords:Cathepsin  Tandem repeat  Phylogeny
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