脉络宁对SH-SY5Y细胞氧糖剥夺再恢复损伤的保护作用及其机制研究

目的：研究脉络宁注射液对SH—SY5Y细胞氧糖剥夺／再复氧糖（OGI）／R）损伤的保护作用，并探讨其可能的作用机制。方法：体外培养SH-SY5Y细胞，将细胞随机分为正常组、氧糖剥夺模型组和脉络宁组（1．0mL·L^-1），建立体外OGD／R细胞模型。倒置显微镜观察细胞形态；MTT法测定细胞存活率；测定乳酸脱氢酶（LDH）漏出量；Western Blot检测凋亡相关蛋白Bcl-2、Bax蛋白表达的变化。结果：与模型组相比，脉络宁能减轻OGD／R引起的SH-SY5Y细胞的损伤，明显提高细胞存活率（P〈0．05），减少LDH的释放量（P〈0．05），有效抑制Bax蛋白的表达（P〈0．05），上调Bcl-2的表达（P〈0．05）。结论：脉络宁注射液对OGD／R引起的SH-sY5Y细胞损伤有保护作用，其机制可能与影响凋亡相关基因Bcl-2、Bax的表达有关。

Protective Effects and Mechanism of Mailuoning on SH-SY5Y Cells Induced by the Oxygen-Glucose Deprivation and Reoxygenation

Objective： To investigate the protective effect of Mailuoning on SH-SY5Y cells induced by the oxygen-glucose deprivation and reoxygenation, and to probe its possible mechanisms. Methods：/n vitro, SH-SY5Y cells were divided into normal group, model group and Mailuoning group（1.0 mL.L^-1）. The oxygen-glucose deprivasion/reperfution（OGD/R） model was made. The protective effect of Mailuoning were observed by morphological identification, MTT assay, and LDH release, while the expressions of Bcl-2 and Bax were detected by Western Blot. Results： Compared with the model group, Mailuoning could relieve the damage of SH-SY5Y cells and in- crease the survival of the cells, lower their LDH level, and adjust the expression of Bcl-2 and Bax proteins. Conclusion： Mailuoning can protect SH-SY5Y cells fi＇om the injury induced by OGD/R, its mechanism may be related to impact the expression of gene related to cell apoptosis.