| 丹参联合β-榄香烯对肝星形细胞LX-2增殖和凋亡的影响 |
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| 引用本文： | 马力天,任秦有,白杨,李成花,杨建栋,孙世友,周睿,杜乐,郑瑾.丹参联合β-榄香烯对肝星形细胞LX-2增殖和凋亡的影响[J].生物磁学,2013(34):6626-6629. |
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| 作者姓名： | 马力天 任秦有 白杨 李成花 杨建栋 孙世友 周睿 杜乐 郑瑾 |
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| 作者单位： | [1]第四军医大学唐都医院中医科暨中西医结合肿瘤科,陕西西安710032 [2]陕西省中医医院,陕西西安710032 [3]辽宁省海城市65547部队医院,辽宁海城114200 [4]第四军医大学放射医学教研室,陕西西安710032 [5]解放军总医院中医院,北京100853 [6]第四军医大学学员旅,陕西西安710032 |
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| 基金项目： | 国家自然科学基金面上项目（81072973）;省级中医药临床研究课题（1c70）致谢：真诚感谢第四军医大学细胞工程学研究中心边惠洁教授给予LX-2细胞系.感谢军事预防医学系放射医学教研室郭国祯主任,王晋副教授、周艳老师,感谢他们在实验平台、技术等多方面给予的指导和帮助. |
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| 摘 要： | 目的：探讨丹参联合B-榄香烯对肝星形细胞LX-2增殖和凋亡的影响。方法：体外培养肝星形细胞LX-2，分别将丹参（浓度为1、2、4、6、8mg／ml），β-榄香烯（浓度为25、50、100、150、200μg／ml），单独作用于LX-2细胞后24h、48h用CCK-8（CellCountKit-8）法检测细胞的增殖情况，并选取合适的药物浓度（丹参3．6mg／ml，β-榄香烯125μg／ml），然后进行联合用药，加药24h、48h后用CCK-8（Cell CountKit-8）法检测细胞的增殖情况，用流式细胞术检测细胞的凋亡率。结果：OCCK-8法显示丹参（浓度为1、2、4、6、8mg／ml），8-榄香烯（浓度为25、50、100、150、200μg／ml）作用LX-2细胞24h、48h后，其增殖抑制率均明显高于正常对照组（P〈0．05），并且呈浓度和时间依赖性。②联合用药（丹参3．6mg／ml，β-榄香烯125μg／ml）时，LX-2细胞的增殖抑制率和凋亡率均显著高于单独用药（P〈0．01）。结论：丹参、β-榄香烯单独或二者联合作用均能抑制LX-2细胞的增殖，且联合应用的作用显著高于单独用药，可协同促进LX-2细胞的凋亡。
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| 关 键 词： | 榄香烯 丹参 LX-2细胞 增殖 凋亡 |
Effect of β-Elemene Combined with Radix Salviae Miltiorrhizae on the Proliferation and Apoptosis of LX-2 Cell |
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| MA Li-tian,REN Qin-you,BAI Yang,LI Cheng-hua,YANG Jian-dong,SUN Shi-you,ZHOU Ru,DU Le,ZHENG Jin.Effect of β-Elemene Combined with Radix Salviae Miltiorrhizae on the Proliferation and Apoptosis of LX-2 Cell[J].Biomagnetism,2013(34):6626-6629. |
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| Authors: | MA Li-tian REN Qin-you BAI Yang LI Cheng-hua YANG Jian-dong SUN Shi-you ZHOU Ru DU Le ZHENG Jin |
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| Institution: | 1 Department integrated traditional and western medicine of ontology, Tangdu Hospital, The Fourth Military Medical University, 710032, Xi＇an, China; 2 Shaanxi TCM Hospital, Xi＇an, Shaaaxi, 710032, China; 3 65547Hospital of PLA, Haicheng, Liaoning, 114200, China; 4 Department of Radiation Medicine, The Fourth Military Medical Universi(y, Xi＇an, 710032, China; 5 Depatement of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing, 100853, China; 6 student teams, The Fourth Military Medical Universi(y, Xi＇an, Shaanxi, 710032, China) |
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| Abstract: | Objective： To investigate the effect of β-Elemene combined with Radix Salviae Miltiorrhizae on the proliferation and apoptosis of LX-2 cell. Methods： Different concentrations ofl3-Elemene injection （25, 50, 100, 150, 200 i~g/ml） and Radix Salviae Miltiorrhizae（1, 2, 4, 6, 8 mg/ml） were solo applied to LX-2 cells. Cell proliferation was measured by CCK-8（Cell Count Kit-8）. The appropriate drug concentration（3.6 mg/ml Radix Salviae Miltiorrhizae, 125 μg/ml β-Elemene） were selected. Then, β-Elemene combined with Radix Salviae Miltiorrhizae injection was applied to LX-2 cells. Cell proliferation was measured by CCK-8 （Cell Count Kit-8）, cell apoptosis was detected by flow cytometry. Results： （L）Cell proliferation were dramatically inhibited by β-Elemene injection （25, 50, 100, 150, 200 μg/ml） and Radix Salviae Miltiorrhizae（1, 2, 4, 6, 8 mg/ml） at 24 h, 48 h （P〈0.05）. In addition, Cell proliferation were dramatically inhib- ited by injection β-Elemene combined with Radix Salviae Miltiorrhizae. ②Combination therapy, apoptosis rate and inhibition rate of proliferation in LX-2 cells treated by β-Elemene combined with Radix Salviae Miltiorrhizae were higher than the monotherapy. Conclusion： Salvia miltiorrhiza, β-Elemene and Salvia miltiorrhiza combined with elemene can inhibit the proliferation, accelerate the apoptosis in LX-2 cells. What＇s more, Salvia miltiorrhiza combined with β-Elemene have synergistic effect on the apoptosis in LX-2 cells, |
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| Keywords: | Elemene Radix Salviae Miltiorrhizae LX-2 cell Proliferation Apoptosis |
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