染色体畸形变与膀胱癌发生发展的相关性研究

目的：通过荧光原位杂交技术（FISH）结合病理分级，探讨染色体畸形变与膀胱癌发生和发展的关系。方法：采用3、7、9、17号染色体着丝粒探针和9P16区带探针对105例膀胱癌复发患者尿液脱落细胞进行荧光原位杂交．观察膀胱癌复发患者中3、7、9、17号染色体的畸形变情况并分析其与患者临床和病理特征之间的关系。结果：105例膀胱癌复发患者中，3、7、9和17号染色体的非整数倍突变率分别是21．9％、29．5％、12．4％、和36．2％，与患者的性别、年龄无显著相关性（P〉0．05）。仅7号染色体畸变与膀胱癌的病理分级具有显著相关性（P〈0．05）。结论：7号染色体畸形变与复发膀胱癌的病理分级显著相关。

Correlation of the Chromosomal Abnormalities with the Occurrence and Development of Bladder Cancer

Objective： To explore the correlation of chromosome abnormalities changes with bladder cancer occurrence and deve- lopment by fluorescence in situ hybridization （FISH） combined with pathological grade. Methods： 3,7,9,17 chromosome centromeric probes and 9P16 zone probe 105 cases of bladder cancer recurrence in patients with urine exfoliated cells by fluorescence in situ hybridization, the 3,7,9,17 chromosome abnormal change in the patients with bladder cancer recurrence and the relationship between the clinical and pathological features were analyzed. Results： In 105 cases of recurrent bladder cancer, the non-integer multiple mutation rates of 3,7,9 and 17 chromosome was 21.9%, 29.5%, 12.4%, and 36.2%, respectively, which had no correlation with the patient＇s gender, age （P〉0.05）. Only the aberrations of chromosome 7 was significantly correlated with the pathological grade of recurrent bladder cancer （P〈0.05）.Conclusion： The aberrations of chromosome 7 was significantly correlated with the pathological grade of recurrent bladder cancer.