生物衍生松质骨支架材料的Micro—CT量化分析

目的：在骨组织工程中，如何制备出理想的支架材料一直是研究重点；目前主要的有天然生物支架材料、人工合成有机材料和无机材料等；生物衍生骨即天然生物支架材料的一种，由于其与天然骨在形态结构上较为相似，是近年来研究较多的支架材料之一；既往形态学研究局限于在二维层面，对于其三维结构参数分析较少。故本实验主要运用Micro-CT对生物衍生松质骨的三维结构参数进行分析，量化评价其作为骨组织工程支架材料的结构参数。方法：截取新鲜猪松质骨，经脱脂脱蛋白部分脱钙及去抗原处理后，制作成生物衍生骨支架；应用Micro．CT扫描，重建三维图并量化分析其结构参数，统计软件SPSS分析各参数间的相关性。结果：经Micro．CT扫描，得到二维CT图和三维重建图。各三维结构参数的值分别为：BV／TV（20．48±5．14）％；BS／BV（41．66±5．39）1／ram；Porosity（79．52±5．14）％；Tb．Th（0．10±0．01）mm；Tb．N（1．99±0．47）l／mm；Tb．Sp（0．32±0．05）mm；Tb．Pf（2．03±4．70）1／mm；SMI（1．28±0．35）；DA（1．60±0．23）；Corm．Dn（158．53±106．09）I／mm3。各参数间相关系数具有统计学意义的为：（1）Porosity与BS／BV、Tb．Th；（2）BV／TV与BS／BV、Tb．Th；（3）BS／BV与Coma．Dn、Porosity、BV/TV、；（4）Tb．Th与Porosity、BV厂IV、Conn．Dn；（5）DA与Corm．Dn；（6）Conn．Dn与Bs／BV、Tb．Th、DA。结论：Micro-CT扫描、量化分析是评价支架材料结构参数的理想方法；也证明生物衍生骨支架符合骨组织工程对支架材料的三维结构要求，尤其在孔径大小、孔隙率、表面积体积比等三维结构参数，此外，也可为其他支架材料的制备在三维结构上的要求提供参考依据。

Quantitatively Analyze Bio-Derived Cancellous Bone Scaffolds Using Micro-CT＊

Objective： How to prepare the ideal scaffold has been a focal point in the bone tissue engineering. At present, the most used scaffolds are natural scaffolds, synthetic organic and inorganic scaffolds. The bio-derived cancellous bone scaffold is one of the natural scaffold materials. Because of its natural bone morphology, the bio-derived cancellous bone scaffold has been one of the research hotspots recently. Previous morphological study is limited to the two-dimensional level, and there are not too much research about the three-dimensional structure. So in the study, micro-CT was used to quantitatively analyze the three dimensional structure of bio-derived cancellous bone scaffolds, and to evaluate its structural parameters as bone tissue engineering scaffolds. Meflaods： The bio-derived bone scaffolds were obtained from fresh porcine cancellous bone after removing fat and protein. Micro-CT was used to scan and quantitatively analyze its structural parameters. The correlation coefficients were calculated by using SPSS statistical software. Results： The bio-derived bone scaffolds were obtained after derosination and deproteinization. 2D and 3D images were gotten through Micro-CT scan. The results of parameters were： BV/TV （20..48± 5.14） %; BS/BV （41.66± 5.39） 1/mm;Porosity （79.52± 5.14） %; Tb.Th （0.10± 0.01） ram; Tb.N （1.99：1： 0.47） 1/mm; Tb.Sp（0.32± 0.05） mm; Tb.Pf（2.03± 4.70） l/mm; SMI（1.28：t 0.35）; DA（1.60± 0.23）; Conn.Dn（158.53± 106.09） 1/mm3. The statistically significant correlation coefficients were： （1）Porosity with BS/BV,Tb.Th; （2）BV/TV with BS/BV, Tb.Th; （3） BS/BV with Conn.Dn, Porosity and BV/TV; （4）Tb.Th with Porosity, BV/TV and Conn.Dn; （5）DA with Conn.Dn; （6）Corm.Dn with BS/BV, Tb.Th and DA. Conclusions： Micro-CT scanning and quantitative analysis was an ideal method to analyze scaffolds structure; It also proved that bio-derived bones scaffold meet the three-dimensional structure of bone tissue engineering requirements, especially in the pore size, porosity, bone surface/volume ratio. In addition, It could provide a reference for another scaffold preparation in the three-dimensional structure.