苦参碱对鼻咽癌细胞凋亡及凋亡相关基因p53的表达研究

目的：探讨苦参碱对体外培养的人鼻咽癌细胞增殖、凋亡及凋亡相关基因p53 mRNA和蛋白表达的影响,初步探讨苦参碱诱导人鼻咽癌细胞凋亡的可能机制。方法：采用MTT法检测不同浓度苦参碱（0、0.25、0.5、1、1.5、2 mg/ml）对CNE1、CNE2细胞增殖的影响;采用荧光定量PCR法检测这些浓度的苦参碱处理48 h后CNE2细胞p53 mRNA的变化;Western Blot检测其蛋白的变化情况。结果：MTT结果显示苦参碱具有抑制CNE1、CNE2细胞体外增殖作用,其抑制率存在浓度、时间依赖性。荧光定量PCR及Western Blot检测结果显示,苦参碱抑制CNE2细胞p53 mRNA和蛋白的表达,且亦呈浓度依赖性。结论：苦参碱抑制CNE2细胞的增殖,诱导细胞凋亡,呈现浓度、时间依赖性,其作用与抑制CNE2细胞中p53基因和蛋白的表达密切相关。

Effects of Matrine on the Apoptosis and the Apoptosis- Related-Gene p53 Expression in Human Nasopharyngeal Carcinoma Cell Line in Vitro

Objective： This study aims to evaluate the effects of matrine on human nasopharyngeal carcinoma cell line proliferation, apoptosis and apoptosis-related-gene p53 expression in vitro and explore the mechanism of conceivable apoptosis promoted by matrine. Methods： In vitro experiments, MTT assay was used to detect the effect of matrine on CNE2 cell proliferation. The expression levels of p53 gene were detected by real-time PCR and western blot analysis. Results： MTT results showed that matrine inhibited CNE2 cells proliferation in a dose and time-dependent fashion. The expression of p53 mRNA was significantly decreased in a dose-dependent fashion （P〈0.01）. The expression of p53 protein was inhibited in a dose-dependent fashion （P〈0.01）. Conclusion： Matrine inhibits CNE2 cells proliferation and induces apoptosis in a dose- and time-dependent fashion, and the decreasing expression of p53 mRNA and protein may closely relate to the mechanism.