电针不同穴位对慢性炎症痛模型大鼠心理行为改变的实验观察

目的：观察电针不同穴位对慢性炎症痛模型大鼠行为学改变的影响，进一步探讨电针对慢性痛所致心理行为改变的调节规律：方法：将40只雄性Wistar大鼠随机分为正常组、模型组、电针百会组、电针关元组、电针足三里组，每组各8只。除正常组外，其余各组均采用弗氏完全佐剂（CFA）制备佐剂性关节炎（AA，即慢性炎症痛）大鼠模型，于造模第2天各治疗组捆绑固定后给予电针20min，正常组及模型组捆绑束缚20min，隔日一次，共10次。于造模前、造模后、治疗5次、10次分别采用高架十字迷宫检测各组大鼠开放臂进入次数比例（OE％）及开放臂停留时间比例（OT％）的变化。结果：与造模前比较，电针百会穴组大鼠造模后0E％和0T％明显减少（P〈0．05，P〈0．05），且治疗5次后OT％、治疗10次后0E％与0T％均降低明显（P〈0．01，P〈0．01，P〈0．05），而电针关元组大鼠治疗10次后OE％和OT％则明显升高（P〈0．05，P〈0．05），电针足三里组大鼠造模后0T％、治疗5次后大鼠OE％与OT％、治疗10次后OT％均明显降低（P〈O．01，P〈0．05，P〈0．01，P〈0．05）；与造模刚结束比较，电针百会组大鼠在治疗5次、10次后0E％由升高逐步降低，而OT％由低逐渐升高（P〉0．05），电针关元组模型大鼠治疗10次后模型大鼠0E％和0T％均明显升高（P〈0．05，P〈0．05），电针足三里组模型大鼠治疗10次后OT％升高明显（P〈0．05）；与治疗5次后比较，电针百会组大鼠在治疗10次后OE％降低、OT％升高（P〉O．05），电针关元组与电针足三里组大鼠治疗10次后OE％和OT％均明显升高（P〈0．05，P〈0．05；P〈0．01，P〈0．01）。与电针百会组比较，电针关元组与电针足三里组大鼠在治疗10次时OE％均明显升高（P〈0．01，P〈0．01）结论：电针不同穴位具有一定抗抑郁和抗焦虑作用，可改善慢性炎症痛所致大鼠焦虑、情绪行为的变化；不同穴位效应不同，关元穴与足三里穴具有综合调节、抗焦虑效应，且具有相对特异性。

Experimental Abservation on Psychological and Behavioral Changes by Electro-Acupuncture at Different Acupoints in Model Rats of Chronic Inflammatory Pain

Objective： To investigate the effect of Electro-Acupuncture at different acupoints on behavioral changes caused by chronic inflammatory pain and to discuss the regulation rule of Electro-Acuptmcture for psychological and behavioral changes caused by chronic pain. Methods： The 40 male Wistar rats were randomly divided into the normal group, the model group, Baihui group, Guanyuan group and Zusanli group, with 8 rats in each group. Except the normal group, the rest rats were used complete Freund adjuvant （CFA） to make adjuvant arthritis （AA, that is chronic inflammatory pain） rat model. The 2nd day after model made, the normal group and the model group were tied up for 20 min, and the treatment groups were used Electro-Acupuncture for 20 min, once every other day, a total of 10 times. All rats were tested into the elevated plus-maze respectively to detect the changes of the proportion of the number of entry into the open arms of the elevated plus-maze （OE %） and that of Residence time in the open arms （OT %） at four time points, including before and after modeling, after the 5th treatment and 10th treatment. Results： Compared with that in the groups before modeling, the OE % and OT % of the rats in Baihui group decreased significantly after modeling （P 〈0.05, P 〈0.05）,and after the 5th treatment the OT % of the rats,after the 10th treatment the OE % and OT % of the rats in Baihui group all decreased significantly （P〈0.01,P〈0.01,P〈0.05）, the OE % and OT% of the rats in Guanyuan group increased significantly after the 10th treatment（P〈0.05, P〈0.05）, after modeling the OT % of the rats.after the 5th treatment the OE % and OT % of the rats,after the 10th treatment the OT % of the rats in Zusanli group all decreased significantly （P〈0.01,P〈0.05,P〈0.01,P〈0.05）; Compared with that in the groups after modeling, the OE % of the rats in Baihui group increased after the 5th treatment and decreased gradually after the 10th treatment, while the OT % of that vice versa （P〉 0.05）, the OE % and OT% of the rats in Guanyuan group increased significantly after the 10th treatment （P〈0.05 ,P〈0.05）, the OT % of the rats in Zusanli group increased significantly after the 10th treatment （P〈0.05）; Compared with that in the groups after the 5th treatment, The OE % of rats in Baihui group decreased and The OT % increased aider the 10th treatment （P〉0.05）,The OE % and OT % of rats in Guanyuan group and Zusanli group all increased significantly after the 10th treatment （P〈0.05, P〈0.05; P〈0.01 ,P〈0.01）. Compared with that in Baihui group, the OE % of rats in Guanyuan group and Zusanli group significantly increased after the 10th treatment （P〈0.01,P〈0.01）. Conclusions： Electro-Acupuncture at different acupoints had some antidepressant and anxiolytic effects, which could improve anxiety and changes in emotion and behavior induced by chronic inflammatory pain; The different acupoints had different effects, Guanyuan and Zusanli having a comprehensive regulation and anxiolytic effect, which had relative specificity.