外源性磷酸肌酸对重度窒息新生儿血清S—100B蛋白及NSE水平的影响

目的：探讨外源性磷酸肌酸对重度窒息新生儿血清S-100B蛋白和特异性神经元烯醇化酶含量（NSE）的影响。方法：重度窒息的新生儿40例，随机分为两组，常规治疗组21例，给予一般治疗（氧疗、支持、对症）和胞二磷胆碱治疗。磷酸肌酸治疗组19例，在常规治疗基础上，生后12h内给予磷酸肌酸治疗lg／d），另外同期住院的新生儿湿肺和黄疸患儿14例为正常对照组。均与生后48h和生后10天取血检测血清S-100B蛋白和NSE含量。并于生后第14天进行新生儿行为神经测定（NBNA评分）。结果：磷酸肌酸治疗组和常规治疗组患儿生后48h血清s．100B蛋白和NsE含量无显著差异（※P〉0．05，※P〉0．05），与正常对照组比较差异具有显著性（△P〈0．05，AP〈0．05），生后10天血清S．100B和NSE含量在常规治疗组患儿和磷酸肌酸组相比具有显著差异，磷酸肌酸治疗组两者明显下降（※P〈0．05，※P〈0．05）。生后三周的行为神经评估（NBNA评分）〈35分者所占百分比磷酸肌酸治疗组27％与常规治疗组组53％比较，差异均具有显著性意义（x2=6．112，※P〈0．05）。结论：磷酸肌酸用于治疗新生儿缺氧缺血性脑病能够改善脑的能量代谢，降低脑损伤的程度，改善神经行为，降低致残率。

Effects of Exogenous Phosphocreatine on Level of S- 100B Protein and NSE in Serum of Newborn with Serious Asphyxia

Objective： To observe the effect of exogenous phosphocreatine on the level changes of S100b protein and NSE In serum with serious hypoxia -ischemic encephalopathy in newborn infants. Methods： 40 serious asphxia newborn were randomly devided into two groups： routin therapy group（21 newborns）, routin treatment with oxygen ,nutrition and citicoline（CTL）.The treatment group（19 newborns） were given exogenous phosphocreatine（12h after born, lg/d） besides conventional therapy which was given in control only.the control group （14 newborn）without asphyxia newborns.The content of S100b protein and NSE in serum were measured using enzyme-linked immuradsorbent assay （ELISA） after 48h and 10 days.The newborns in both groups were assessed with neurological function, the NABA scores was recorded. Results： The level of NSE and S100B protein In serum in the treatment group and routin theray group in 48h were no significantly difference （ ※ P 〉 0.05, ※ P 〉 0.05 ）.As compared with control group it was significantly difference （ △P〈0.05,△P〈0.05 ）. The level of NSE and S-100B protein In serum after 10 days between two group were significantly difference, the treatment group were significantly decreased （※P〈0.05, ※P〈0.05）. The NBNA score〈 35 point after 3 weeks account for 27.0% in treatment group and 53% in control group,which had significantly difference （x2 =6.112, ※ P〈0.05）. Conclusions： exogenous phosphocreatine can protect brain tissure and improve the energetic metabolism of the brain cell, can lessen cerebral ischemic injury, which can decrease disability rate and improve neurological function.