大鼠急性肾缺血再灌注损伤模型的建立与评估

目的：对现有的经腹部切口建立急性肾缺血再灌注损伤动物模型进行改良，探索建立急性肾缺血再灌注损伤模型的新方法。方法：实验组大鼠16例，经背部切口进入腹膜后间隙，游离钳夹双侧肾动脉45min后开放血流，建立急性肾缺血再灌注损伤模型；伪手术组8例，不夹闭肾动脉，余步骤与实验组相同；对照组8例无处理。术后通过建模成功率、组织病理检查、血肌酐和血尿素氮及氧化应激水平对模型进行评估。结果：实验组15只成功建立急性肾缺血再灌注损伤模型。术后1天病理检查显示实验组肾组织出现广泛损伤，术后实验组’肾小管坏死评分、肾MDA水平、血肌酐及血尿素氮值明显高于对照组（P〈0．05）。结论：经背部切口钳夹双侧肾动脉可建立稳定的大鼠急性肾缺血再灌注损伤模型。该造模方法简便易行，成功率高，且具备手术切口小、手术时间短及并发症少的优点，建立的模型适合于急性肾损伤的研究。

Establishment and Evaluation of Rat Acute Kidney Ischemia/Reperfusion Model

Objective： To improve current acute kidney ischemiaJreperfusion animal model through abdominal incision and investigate a new approach to establish acute kidney ischemia/reperfusion animal model. Methods： Acute rat kidney ischemia/reperfusion model was established by dorsal incision by clamping bilateral renal arteries for 45 min in experimental group （n=16）; The same procedure without renal artery clamping was implemented in sham operating group （n=8）; No treatment was given to control group （n=8）. Established model was assessed by histopathological examining, concentrations of serum creatinine and blood urea nitrogen, and oxidative stress in kidney and success ratio of acute kidney ischemia/reperfusion model establishment. Results： Acute kidney ischemia/reperfusion model was established successfully. There were extensive injuries in experimental group, while kidney morphostructure appeared normal in control group. Tubular injury score, MDA level, concentrations of serum creatinine and blood urea nitrogen in experimental group after surgery were significantly higher than those in control group （P〈0.05）. Conclusions： Acute rat kidney ischemia/reperfusion model can be established steadily by clamping bilateral renal arteries via dorsal incision. This procedure is simple, convenient and of high success ratio, and have the advantages of smaller operative incision, shorter operation time and fewer complications after surgery, by which it is suitable for in-depth research based on acute kidney injury.