小茴香对肝纤维化大鼠脂质过氧化水平的影响

目的：研究小茴香对肝纤维化大鼠的脂质过氧化水平的影响。方法：取Wister大鼠100只，随机取12只设为正常组，其余采用四氯化碳（CCL4）复合因素法复制肝纤维化大鼠模型，复制成功后随机分为模型对照组、小茴香组和复方丹参组（n=12）；相应药物干预8周后，镜检肝组织形态学改变；全自动生化分析仪检测外周血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平与血清总蛋白（TP）、白蛋白（ALB）含量；生化法检测肝组织羟脯氨酸（HYP）与血清丙二醛（MDA）含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—PX）、过氧化氢酶（CAT）活性。结果：HE结果显示：模型组肝脏炎症与脂肪变性明显，经小茴香干预后明显改善。生化检测结果显示：与正常组比较，模型组ALT、AST水平显著升高（P〈0．05），TP、ALB含量显著降低（P〈O．05），HYP、MDA含量显著增加（P〈0．05），而SOD、GSH—PX和CAT活性显著降低（P〈0．05）；与模型组比较，小茴香组ALT、AST水平显著下降（P〈0．05），TP、ALB水平显著升高（P〈0．05）；HYP、MDA含量显著降低（P〈0．05），SOD、GSH—PX和CAT活性显著提高（P〈0．05）。结论：小茴香的抗肝纤维化作用可能与调节大鼠脂质过氧化水平有关。

Effects of foeniculum vulgare mill on lipid peroxidation in rats with liver hepatic fibrosis

Objective： To observe the effects of foeniculum vulgare mill on liver fibrosis. Methods： 100 Wister rats were randomly divided into 4 groups as control group ,model group, foeniculum vulgare mill group, and danshen group. Liver fibrosis models were established by the combination of injecting carbon tetrachloride （CCL4） subcutaneously. Drugs were lavaged for 8 weeks, rats livers tissue were examined with HE staining; the serum level of alanine transaminase （ALT）, aspartate aminotransferase（AST）, albumin（ALB） and total protern （TP） were determined using corresponding test kits; the hepatic lipid peroxidation indexes, including malondialdehyde （MDA）, Superoxide dismutase（SOD）,Glutathione peroxidase（GSH-PX）,Catalase（CAT） were measured by biochemical methods; Results： Compared with normal group, ALT, AST level and MDA content remarkablely increased, level of TP, ALB and SOD activity decreased （P〈0.05）. Compared with model group, Foeniculum Vulgare Mill group ALT, AST level and MDA content remarkablely decreased, level of TP, ALB and SOD, GSH-PX, CAT activity remarkablely increased （P〈0.05）. Conclusions： Foeniculum Vulgare Mill can inhibit the hepatic fibrosis and it＇s probably mechanism is associated with the effect of Foeniculum Vulgare Mill regulate the lipid peroxidation in the liver.