| DETs缓冲液对肠道微生物DNA的保存效果 |
| |
| 引用本文： | 蔡重阳,张于光,李迪强,任佐华,刘学端.DETs缓冲液对肠道微生物DNA的保存效果[J].生物磁学,2009(13):2484-2486. |
| |
| 作者姓名： | 蔡重阳 张于光 李迪强 任佐华 刘学端 |
| |
| 作者单位： | [1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林生态环境重点实验室,北京100091 [2]湖南农业大学生物安全科技学院,湖南长沙410128 |
| |
| 基金项目： | 国家科技部科技基础条件平台建设项目（2005DKA21404）,科技基础性工作专项（2006FY110800-07） |
| |
| 摘 要： | 方便高效的保存方法是开展动物肠道微生物研究的重要前提,用T-RFLP（末端限制性片段长度多态性）结合16S rDNA分析评估了野外采样常用的DETs（20%DMSO,0.25 M sodium-EDTA,100 mM Tris,pH 7.5,and NaCl to saturation）缓冲液对不同保存时间（24h,7天,30天,90天）粪便样品肠道微生物DNA的保存效果。结果表明,DETs保存在30天保存时间内微生物群落结构无显著变化,95.6%的T-RFs仍然存在,能效好的保护肠道微生物DNA。
|
| 关 键 词： | T-RFLP 16SrDNA 微生物群落 肠道微生物 保存方法 |
Effectiveness of DETs Buffer for Intestinal Microbes DNA Preservation |
| |
| CAI Chong-yang,ZHANG Yu-guang,LI Di-qian,REN Zuo-hua,LIU Xue-duan.Effectiveness of DETs Buffer for Intestinal Microbes DNA Preservation[J].Biomagnetism,2009(13):2484-2486. |
| |
| Authors: | CAI Chong-yang ZHANG Yu-guang LI Di-qian REN Zuo-hua LIU Xue-duan |
| |
| Institution: | 1. School of Minerals Processing ＆ Bioengineering of Central South University, Changsha 410083, China; 2 Environment and Protection of Institute of Forestry Ecology of Chinese Academy ofForestry, Beijing 100091, China; 3 College of Biosafety Science and Technology of Hunan Agricultural University, Changsha 410128, China) |
| |
| Abstract: | Convenient and effective preservative methods are important to intestinal microbes community research. We evaluated the effectiveness of DETs buffer （20% DMSO, 0.25 M sodium-EDTA, 100 mM Tris, pH 7.5, and NaCI to saturation） for intestinal microbes DNA Preservation. Effectiveness was evaluated by T-RFLP （Terminal restriction fragment length polymorphism） combined with 16S rDNA analysis at time points of 24 hours, 7 days, 30 days, 90 days.The results showed that DETs buffer could preserve samples well for 1 month （95.6% peaks were kept, means）. |
| |
| Keywords: | T-RFLP 16SRdna Microbial community Intestinalmicrobes Preservation method |
| 本文献已被 维普 等数据库收录！ |
|
