硒对H2O2诱导的人甲状腺细胞凋亡和超微结构改变的影响

目的：观察硒对H2O2诱导的人甲状腺上皮细胞凋亡和超微结构改变的影响。方法：取良性甲状腺腺瘤旁正常组织进行细胞培养。加硒（10^-7mol／L）或不加硒后加入不同浓度H2O2（0～800μmol／L）刺激单层培养的甲状腺细胞，流式细胞术（FCM）检测甲状腺细胞凋亡率并在电镜下观察其超微结构的改变.结果：经过H2O2作用24h的人甲状腺细胞，随H2O2浓度升高，细胞凋亡率逐渐升高；电镜下甲状腺细胞超微结构呈损伤型改变，甚至出现凋亡、死亡。预先加入10^-7mol／L硒可降低细胞凋亡率，可明显减轻亚细胞结构损伤。结论：硒可减轻H2O2诱发的人甲状腺细胞的氧化损伤，拮抗其导致的细胞凋亡。

The Effect of Selenium on Apoptosis and Ultrastrucmre of Human Thyrocyte Induced by H202

Objective： To investigate the influence of selenium （Se） on apoptosis and ultrastructure of human thyrocytes induced by H2O2 culture in vitro. Methods： The human thyroid epithelium cells （TEC） from paraadenoma normal tissues of patients with thyroid adenorna were cultured. The H2O2 at different concentration （0-800μmol/L）was added into thyrocyte of monolayer culture after without or with Se （10^-7mmol/L）. The thyrocyte apoptosis rates were detected using flow cytometry. And the thyrocyte ultrastructure of each group were observed. Results： When the thyrocyte was exposed to H2O2 for 24 hours, the cell apoptosis rates gradually increased with the increased concentration of H2O2. Under the TEM, damaging changes were observed at thyrocytes when the thyrocyte was exposed to H2O2 for 24 hours. Se at the concentration of 10^-7mmol/L was added into cell culture, and it could effectively reduce the thyrocyte apoptosis caused by H2O2. Selenium preintervention in each group can reduce the injury of cell ultrastructure significantly. Conclusions： Selenium could protect thyroid follicular epithelial cell against oxidative damage and apoptosis caused by H2O2.