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西比灵干预对沙鼠脑缺血再灌注后核因子-κB、单核细胞趋化蛋白-1表达的影响
引用本文:朱勇,胡治平.西比灵干预对沙鼠脑缺血再灌注后核因子-κB、单核细胞趋化蛋白-1表达的影响[J].生物磁学,2009(13):2526-2527,F0003.
作者姓名:朱勇  胡治平
作者单位:[1]株洲市一医院神经内科,株洲412000 [2]中南大学湘雅二医院神经内科,长沙,410008
基金项目:国家自然科学基金资助项目(30470602)
摘    要:目的:研究西比灵对沙鼠脑缺血再灌注后核因子-κB、单核细胞趋化蛋白-1表达的影响。方法:52只健康蒙古沙鼠随机分为正常对照组、假手术组、脑缺血再灌注(I/R)组、西比灵干预组,I/R组及西比灵干预组再分为6h、1d、3d、7d四个亚组。通过夹闭双侧颈总动脉10 min后松夹,建立沙鼠全脑缺血再灌注模型。采用免疫组化的实验方法检测各组脑组织中NF-κBp65、MCP-1的表达。结果:缺血再灌注后各时间点I/R组及西比灵干预组,NF-κBp65的表达量显著高于正常对照组及假手术组(均P〈0.01),且出现MCP-1阳性表达。与I/R组比较,西比灵干预组在I/R后6h、1d,NF-κBp65、MCP-1表达下调(均P〈0.05)。结论:在脑缺血再灌注早期,西比灵能够下调NF-κBp65、MCP-1的表达,减轻局部炎症反应及脑缺血再灌注损伤。

关 键 词:西比灵  脑缺血再灌注  NF-κB  MCP-1

The effect of flunarizine on their expressions of NF-κB and MCP- 1 in ischemic brain tissue following cerebral ischemia/reperfusion in gerbils
ZHU Yong,HU Zhi-Ping.The effect of flunarizine on their expressions of NF-κB and MCP- 1 in ischemic brain tissue following cerebral ischemia/reperfusion in gerbils[J].Biomagnetism,2009(13):2526-2527,F0003.
Authors:ZHU Yong  HU Zhi-Ping
Institution:1 First Hospital of Zhuzhou, Zhuzhou, 412000, China; 2 Second Xiangya Hospital, Central South University, Changsha 410011, China )
Abstract:Objective: To investigate the effect of flunarizine on the expressions of NF-κB and MCP-1 in brain tissue following cerebral ischemia/reperfusion in gerbils. Methods: Fifty-tow healthy gerbils were randomly divided into four groups: normal control group, sham operated group, ischemia/reperfusion (UR)group and flunarizine intervention group. I/R group and flunarizine intervention group were subdivided into four reperfusion subgroups for 6h, 1d, 3d and 7d. The model of pan-cerebral ischemiaJreperfusion was established by blocking bilateral common carotid artery with non-injury artery clap for 10 minutes. The expressions of NF-κBp65 and MCP-1 were detected with immunohistochemistry method. Results: At 6h-7d post-I/R, the level of NF-κBp65 in I/R group and flunarizine intervention group was significantly higher than that of the normal control group and sham operated group (P〈0.01), and the MCP-1 was explored. The expressions of NF-κB65 and MCP-1 were lower in flunarizine intervention group than in lfR group at the same time, which were in reperfusion for 6h and 1d respectively(P〈0.05). Conclusions: During the early phase of cerebral ischemia/reperfusion, flunarizine can down-regulate the expressions of NF-κBp65 and MCP-1, and has neuroprotective effect against the local inflammatory reaction and ischemia/reperfusion injury.
Keywords:Flunarizine  Cerebral ischemia/reperfusion  NF-κB  MCP-1
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