肝郁脾虚证模型大鼠血流变及TXB_2、PGFla的变化（英文）

目的：探查中医肝郁脾虚证模型的血流变及相关调节因子的状态。方法：采用慢性束缚应激＋过度疲劳＋饮食失节法建立大鼠肝郁脾虚证模型,测定大鼠造模三周、自然恢复一周时的血流变和血浆TXB2、PGF1a。结果：与正常组相比,模型组大鼠造模三周150/s、38/s、10/s、5/s切变率下的全血粘度、还原粘度均显著升高（P〈0.001）,红细胞聚集指数显著降低（P〈0.001）,红细胞压积显著升高（P〈0.01）,红细胞变形指数无显著性差异（P〉0.05）;血浆TXB2显著升高（P〈0.001）,6-keto-PGF1a显著降低（P〈0.05）,TXB2/PGF1a显著升高（P〈0.01）;模型组大鼠第四周150/s、38/s、10/s、5/s切变率下的全血粘度、还原粘度仍显著升高（P〈0.001或P〈0.01）;红细胞聚集指数显著降低（P〈0.001）;红细胞压积与变形指数无显著性差异（P〉0.05）;血浆TXB2和TXB2/PGF1a显著降低（P〈0.05）,6-keto-PGF1a显著升高（P〈0.05）。结论：肝郁脾虚证大鼠存在血液高粘和血栓易形成状态,恢复期血液高粘同时伴有扩血管因素的加强。提示肝郁脾虚证有血流变的异常和血浆TXB2-PGI2的平衡失调,主要涉及到血小板和血浆因素的参与。

Change of Hemorheology and TXB_2 and PGF1a in the Blood of the Rat with Liver-qi Stagnation and Spleen-qi Deficiency Syndrome

Objective：To investigate the change of hemorheology and TXB2 and PGF1a in the TCM＇s syndrome model of GYPX（stagnation of liver-qi and deficiency of spleen-qi）made by a new compound method.Methods：Adopting chronic astriction,excess fatigue and improper diet on rats for constant three weeks and stopping treatment on them at the 4th week,determining the hemorheology and the contents of TXB2 and PGF1a in plasma of the rat of each group.Results：Compared with the normal group,the whole blood viscosity and reduced viscosity under different shear rates of model group＇s rats increased significantly（P0.001） at the 3rd week;The index of erythrocyte aggregation decreased（P0.001）,hematocrit increased significantly（P0.01） and the index of erythrocyte deformability had no change.The TXB2 and TXB2/PGF1a in plasma of model group＇s rats increased significantly（（P0.001或P0.01） and 6-keto-PGF1a decreased（P0.05） at the 3rd week;The whole blood viscosity and reduced viscosity under different shear rates of model group＇s rats still increased significantly（P0.001or P0.01） at the 4th week;The index of erythrocyte aggregation decreased significantly（P0.001）;But the hematocrit and the index of erythrocyte deformability had no any changes（P0.05）.The TXB2 and TXB2/PGF1a of the 4th week were lower than those of the 3rd week,and decreased significantly compared with normal group（P0.05）;The 6-keto-PGF1a was higher than that of the 3rd week,and increased significantly compared with normal group（P0.05）.Conclusion：The GYPX model of the rats is in a state of high viscosity and easy to form thrombus.The model of the GYPX in convalescence is in a state of high viscosity,associated with the strengthened factor of dilatation of the blood vessel.It clues to the abnormal change of hemorheology and malajustment of TXB2,PGF1a in the model of GYPX.These changes perhaps are concerned mainly with the factor of platelet and plasma.