重楼皂甙Ⅱ对狼疮性肾炎患者外周血CD4~＋CD25~＋T调节细胞表达的细胞因子的影响

目的：研究重楼皂甙Ⅱ对狼疮性肾炎患者外周血CD4＋CD25＋Treg分泌的细胞因子IL-10和TGF-β的影响。方法：10例健康人为正常对照组,20例LN病人随机分为四组：LN对照组（n=5）、重楼干预组1（n=5,接种细胞数为1×105/1.5ml/孔）、重楼干预组2（n=5,接种细胞数为2×105/1.5ml/孔）、重楼干预组3（n=5,接种细胞数为5×105/1.5ml/孔）。分离外周血单个核细胞,利用免疫磁珠法分离CD4＋CD25＋Treg。重楼干预组予重楼皂甙Ⅱ干预,正常对照组和LN对照组不予处理,各组培养72h。取各组上清,用ELISA分别检测IL-10和TGF-β的水平。结果：与正常组比较,其余各组TGF-β和IL-10的水平明显降低（P〈0.01）。与LN对照组比较,重楼皂甙Ⅱ干预后各组TGF-β和IL-10的水平升高（P〈0.05）。重楼干预组之间比较,重楼干预组2的TGF-β和IL-10水平较其他两组明显升高（P〈0.01）,而重楼干预组1和3的TGF-β和IL-10水平变化无显著性差异（P〉0.05）。结论：重楼皂甙Ⅱ可上调LN患者TGF-β和IL-10的水平,上调的幅度受接种细胞数量的影响。

The effects of Paridis saponin Ⅱ on the production of cell cytokines in CD4~＋CD25~＋ T regulation cells from lupus nephritis patients＇ peripheral blood

Objective：To investigate the effects of Paridis saponin Ⅱ on the TGF-β and IL-10,which were secreted by CD4＋CD25＋ T regulation cells from the peripheral blood of LN patients.Methods：10 healthy person were composed to the normal control group,and the 20 LN patients were divided into 4 groups randomly：the LN control group（n=5）,the first Paridis saponin Ⅱ group（n=5,added Paridis saponin Ⅱ was 1×105/1.5ml/ pore）,the second Paridis saponin Ⅱ group（n=5,added Paridis saponin Ⅱ was 2×105/1.5ml/pore）,the third Paridis saponin Ⅱ group（n=5,added Paridis saponin Ⅱ was 5 ×105/1.5ml/ pore）.Isolate peripheral blood mononuclear cells（PBMC） and then isolate CD4＋CD25＋ Treg with MACS.Added in Paridis saponin Ⅱ to Paridis saponin Ⅱ groups,and nothing to the normal control group and the LN control group.The 5 groups were cultured for 72 hours.ELISA was used to analyze the level of TGF-β and IL-10 in the supernatant.Results：The level of TGF-β and IL-10 were significant lower in LN groups than those in control group（P0.01）.The level of TGF-β and IL-10 were higher in the groups which were cultured with Paridis saponin Ⅱ than the group without the treatment（P0.05）.Among the groups which were cultured with Paridis saponin Ⅱ,the level of TGF-β and IL-10 of the second group was significant higher than the first and the third（P0.01）.The change of the first and the third was not significant obvious（P0.05）.Conclusion：Paridis saponin Ⅱ can increase the LN patients＇ level of TGF-β and IL-10.The range of increase was influenced by the quantities of the added cells.