| 三硝基苯磺酸法测定聚乙二醇化重组人生长激素的平均修饰度 |
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| 引用本文： | 李晶,王永利,杨化新,梁成罡,程速远,丁晓丽,吕萍.三硝基苯磺酸法测定聚乙二醇化重组人生长激素的平均修饰度[J].生物磁学,2011(9):1609-1612. |
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| 作者姓名： | 李晶 王永利 杨化新 梁成罡 程速远 丁晓丽 吕萍 |
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| 作者单位： | [1]中国食品药品检定研究院,北京100050 [2]宁夏大学生命科学学院,宁夏银川750021 |
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| 基金项目： | ＂重大新药创制＂科技部重大专项课题＂生物技术药物质量标准和质量控制技术平台（2009ZX09307）; 中国药品生物制品检定所中青年发展基金课题（2009A5）资助 |
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| 摘 要： | 目的：建立测定三种不同结构聚乙二醇化重组人生长激素（PEG-rhGH）平均修饰度的三硝基苯磺酸法（TNBS）。方法：采用基质辅助激光解吸附飞行时间质谱（MALDI-TOF）测定PEG-rhGH的分子量,求得理论平均修饰度。既而通过正交试验、游离PEG干扰等方法学考察,建立TNBS法。结果：从质谱法可知PEG-rhGH的理论修饰度为10%,TNBS法测得三种PEG-rhGH的修饰度分别为为10%、12%及10%,与理论值相符。结论：TNBS操作简单,不需要专门的仪器,可作为测定PEG-rhGH平均修饰度的方法。
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| 关 键 词： | 聚乙二醇化重组人生长激素 三硝基苯磺酸法 平均修饰度 |
Determination of Average Degree of Modification of Pegylated Recombinant Human Growth Hormone by Trinitrobenzene Sulfonic Acid |
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| LI Jing,WANG Yong-li,YANG Hua-xin,LIANG Cheng-gang,CHENG Su-yuan,DING Xiao-li,LV Ping.Determination of Average Degree of Modification of Pegylated Recombinant Human Growth Hormone by Trinitrobenzene Sulfonic Acid[J].Biomagnetism,2011(9):1609-1612. |
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| Authors: | LI Jing WANG Yong-li YANG Hua-xin LIANG Cheng-gang CHENG Su-yuan DING Xiao-li LV Ping |
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| Institution: | 1 National Institutes for Food and Drug Control, 100050, Beijing, China; 2 The College of Life Sciences, Ning Xia University, 750021, Yinchuan, China) |
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| Abstract: | Objective： To determine the average extent of modification of PEG-rhGH by the established TNBS method. Methods： MALDI TOF mass spectrometry was used to detecte the molecular weight and the theoretical extent of modification of PEG-rhGH. TNBS method was established by the orthogonal experiment and the interference test of free PEG. Results： The actual modification ex- tent of three kinds of PEG-rhGHs were 10%, 12% and 10% respectively, and corresponding to the theoretical value of the modification extent it was 10%. Conclusions： TNBS method is an efficient, convenient and suitable method for the quality control of PEG-rhGH on the average extent of modification. |
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| Keywords: | Pegylated recombinant human growth hormone Trinitrobenzene sulfonic acid Average extent of PEG modification |
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