首页 | 本学科首页   官方微博 | 高级检索  
   检索      

米诺环素对糖尿病大鼠视网膜神经细胞凋亡的影响
引用本文:练海东,任兵,高晓唯,郭继华,解玉军,张改丽,赵旭东,刘莹.米诺环素对糖尿病大鼠视网膜神经细胞凋亡的影响[J].生物磁学,2011(13):2435-2437.
作者姓名:练海东  任兵  高晓唯  郭继华  解玉军  张改丽  赵旭东  刘莹
作者单位:[1]石河子大学医学院一附院眼科,新疆石河子832000 [2]解放军474医院全军眼科中心,新疆乌鲁木齐830013 [3]解放军474医院病理科,新疆乌鲁木齐830013 [4]石河子大学医学院,新疆石河子832000
摘    要:目的:观察米诺环素对糖尿病大鼠视网膜神经细胞的凋亡的影响,研究米诺环素对糖尿病视网膜神经保护作用,对米诺环素在糖尿病视网膜疾病中抑制神经细胞凋亡提供理论支持。方法:选择健康成年雄性SD大鼠30只,随机分成正常对照组、糖尿病模型组和米诺环素治疗组,每组10只。腹腔内注射链脲佐菌素(STZ)诱发大鼠糖尿病。米诺环素治疗纽给予米诺环素腹腔注射(45mg/kg),共注射10d,模型组和对照组腹腔注射等体积生理盐水,于给药后8周的3组动物处死,冰浴下取其视网膜组织,随后制备视网膜石蜡切片,采用末端脱氧核糖核酸介导生物素化脱氧尿嘧啶缺口末端标记L(TUNEL)法进行凋亡细胞原位标记,行视网膜神经细胞凋亡计数,对所有数据进行统计学分析。实验结果拟用均数和标准差(x±s)表示,P〈0.05为差异有统计学意义。结果 相同观察时相,与阴性对照组比较,模型对照组大鼠视网膜TUNEL阳性细胞显著增多(P〈0.01),在米诺环素组,大鼠视网膜TUNEL阳性细胞数比模型对照组明显减少,差异有统计学意义(P〈0.01)。结论:米诺环素能有效降低糖尿病大鼠视网膜神经细胞的凋亡,对糖尿病视网膜神经细胞有保护作用。

关 键 词:糖尿病视网膜病变  米诺环素  凋亡

Effects of Minocycline on Apoptosis of Retinal Nerve Cells of Rats with Diabetic Retinopathy
LIAN Hai-dong,REN Bing,GAO Xiao-wei,GUO Ji-hua,XIE Yu-jun,ZHANG Gai-li,ZHAO Xu-dong,LIU Ying.Effects of Minocycline on Apoptosis of Retinal Nerve Cells of Rats with Diabetic Retinopathy[J].Biomagnetism,2011(13):2435-2437.
Authors:LIAN Hai-dong  REN Bing  GAO Xiao-wei  GUO Ji-hua  XIE Yu-jun  ZHANG Gai-li  ZHAO Xu-dong  LIU Ying
Institution:1Department of Ophthalmology, The First Affiliated Hospital of Medical college, shihezi University, Shihezi, Xinjiang ,832000, China; 2 Ophthalmic Center, NO.474 Hospital of Chinese PLA, Urumuqi,xinjiang, 830013, China)
Abstract:Objective: To study the effect of the Minocycline (MNC) on expression of apoptosis in retinal nerve cells of rat with diabetic retinopathy (DR). Methods: A total of 30 male SD rats were randomly divided into negative control group,model control group and MNC treated group. Diabetes model was established by intraperitoneal injection of 60 mg/kg streptozotocin (STZ). Terminal Deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL staining) was used to observe neuron apoptosis in retina. All the experimental data were expressed by Mean+standard deviation. Results: Compared with the negative control group, TUNEL positive cells in retina were increased significantly (P〈0.01) in model control group. However, TUNEL positive cells in retina in MNC treated group were significantly decreased (P〈0.01). Conclusion: MNC can obviously decrease the expression ofapoptosis in retina,with DR. It is one of path of inhibiting impairment on retinal nerve cells with DR.
Keywords:Diabetic retinopathy  Mincycline  Apoptosis
本文献已被 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号