| 基于核酸侵入反应的生物分子检测技术的研究进展 |
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| 引用本文： | 马寅姣,邹秉杰,王建平,周国华.基于核酸侵入反应的生物分子检测技术的研究进展[J].生物磁学,2011(12):2384-2388. |
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| 作者姓名： | 马寅姣 邹秉杰 王建平 周国华 |
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| 作者单位： | [1]中国药科大学生命科学与技术学院,江苏南京210009 [2]华东医学生物技术研究所,江苏南京210002 |
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| 基金项目： | 江苏省自然科学基金（No.BK2010111）; 国家自然科学基金项目（No.21005088） |
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| 摘 要： | 核酸侵入反应是由5＇核酸内切酶或flap内切酶催化的,能够识别切割核酸片段形成的特异性结构的一类反应。近年来发展了很多基于该反应的生物大分子检测技术,能够对DNA、RNA、miRNA及蛋白质进行高灵敏、高特异性的测定。这些技术大都无需扩增待测靶标,极大地降低了扩增产物交叉污染的风险,在临床检测中具有很大的应用前景。本文对这些检测技术的原理及应用作简要综述。
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| 关 键 词： | 核酸侵入反应 5＇核酸内切酶 Flap内切酶 信号扩增 |
Advances in Bio-molecular Diagnostic Assays Based on Invasive Reaction |
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| MA Yin-jiao,ZOU Bing-jie,WANG Jian-ping,ZHOU Guo-hua.Advances in Bio-molecular Diagnostic Assays Based on Invasive Reaction[J].Biomagnetism,2011(12):2384-2388. |
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| Authors: | MA Yin-jiao ZOU Bing-jie WANG Jian-ping ZHOU Guo-hua |
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| Institution: | 1 School of life science and technology,China Pharmaceutical University,Nanjing 210009,China; 2 Huadong Research Institute for Medicine and Biotechnics,Nanjing 210002,China) |
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| Abstract: | The invasive reaction is catalyzed by 5＇ endonuclease or flap endonuclease,which can recognize and cleavase a specific structure formed by oligonucleotides.Recently,numerous newly techniques based on invasive reaction have been developed to detect biological molecules,such as DNA,RNA,miRNA and protein,with a high sensitivity and specificity.These techniques do not depend on target amplification,so the risk of cross-contamination decreased significantly,and it show great potential in clinical diagnostics.This article gives a review on the principles and applications of these developed assays. |
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| Keywords: | Invasive reaction 5＇ Endonuclease Flap endonuclease Signal amplification |
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