系统性红斑狼疮患者血清中CD83与自身抗体的表达及其相互关系

目的：检测系统性红斑狼疮（systemic lupus erythematosus,SLE）患者血清中CD83（soluble CD 83,sCD 83）和多种自身抗体的表达水平,并探讨其相互关系。方法：ELISA检测患者可溶性CD 83和AnuA的表达,应用间接免疫荧光的方法检测抗cmDNA抗体,应用乳凝法检测血清中的DNP,采用胶体金标记和快速膜渗滤技术测定血清中的抗dsDNA抗体。结果：对照组患者血清中可溶性CD 83的表达为（0.26±0.10）ng/ml,实验组患者血清中可溶性CD 83的表达为（5.56±0.72）ng/ml。与对照组相比,实验组患者血清中可溶性CD 83的平均浓度明显升高。在抗dsDNA抗体阴性的51例系统性红斑狼疮患者中AnuA的阳性率明显高于抗DNP抗体和抗cmDNA抗体,同样在抗DNP抗体阴性的58例系统性红斑狼疮患者中AnuA的阳性率明显高于dsDNA抗体和抗cmDNA抗体。系统性红斑狼疮患者中可溶性CD83的水平（〈2.68 ng/ml）与各种自身抗体（抗dsDNA抗体、AnuA、抗DNP抗体和抗cmDNA抗体）水平的相关系数分别为（r=0.542,0.613,0.489和0.367）。具有高水平可溶性CD83的系统性红斑狼疮患者（≥2.68 ng/ml）,与各种自身抗体（抗dsDNA抗体,AnuA,抗DNP抗体和抗cmDNA抗体）水平的相关系数分别为（r=0.711,P〈0.05）、（r=0.845,P〈0.01）、（r=0.862,P〈0.01）和（r=0.724,P〈0.051）。结论：可溶性CD83通过活化DC细胞并激活补体系统,参与系统性红斑狼疮的发生发展,联合可溶性CD83和多种自身抗体的检测,能更明确系统性红斑狼疮患者病情的严重程度,有利于SLE的诊断和治疗。

Expression and correlation of soluble CD83 and autoantibodies in patients with systemic lupus erythematosus

Objective：To determine the expression of CD83 and autoantibodies in patients with systemic lupus erythematosus（SLE）,and explore their mutual relations.Methods：ELISA was detected the soluble CD 83（sCD 83） and AnuA expression,and indirect immunofluorescence antibody method for detection of anti cmDNA,and the application of serum milk coagulation for the DNP,using colloidal gold labeled membrane filtration technology and rapid determination of serum anti-dsDNA antibodies.Results：In the control group,CD 83 expression is（0.26 ± 0.10） ng / ml,sCD 83 expression is（5.56 ± 0.72） ng / ml in the experimental group.Compared with the control group,sCD 83 expression of experimental group was significantly increased.Anti-dsDNA antibody negative） in 51 patients,AnuA（positive） patients was significantly higher than anti-DNP antibodies and anti cmDNA antibodies.The anti-DNP antibodies（negative） in 58 patients were significantly higher than AnuA and anti-dsDNA antibodies（positive）.The high levels of soluble CD83 in patients（≥ 2.68 ng / ml） were related to a variety of autoantibodies（anti-dsDNA antibodies,AnuA,anti-DNP antibodies and anti cmDNA antibody） levels（r=0.711,P 0.05）,（r=0.845,P 0.01）,（r=0.862,P 0.01） and（r=0.724,P 0.051）.Conclusion：soluble CD83 was through with activation of DC and activate the complement system,involved in the development of systemic lupus erythe-matosus,the United soluble CD83 and a variety of autoantibodies will be more clearly the severity of systemic lupus erythematosus,and conducive to the diagnosis and treatment of SLE.