番茄红素对大鼠乳鼠心肌细胞缺氧复氧的保护作用以及其机制

目的：探讨番茄红素对心肌细胞缺氧复氧的保护作用以及其分子机制。方法：采用原代培养心肌细胞建立缺氧/复氧损伤模型,实验分8组：正常对照组,H/R组,H/R＋番茄红素（1,2,4,8,16,32μmol/L）剂量组。观察各组细胞经H/R损伤后,细胞内天冬氨酸氨基转移酶（AST）、肌酸激酶（CK）、乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量的变化情况,选择正常对照组,H/R组,最佳番茄红素剂量组做MTT分析细胞凋亡,Western检测TRL 4以及NF-κB的表达。结果：番茄红素（16,8,4,2μmol/L）剂量组可显著降低缺氧/复氧损伤心肌细胞内AST、CK、LDH释放量及MDA的生成,并能提高SOD活性。此外番茄红素可减少心肌细胞缺氧/复氧损伤后的心肌凋亡,减少TRL 4受体以及NF-κB的表达。结论：番茄红素具有抗缺氧/复氧损伤,保护心肌细胞的作用,其机制可能是通过抑制TRL 4通路来实现的。

Protective Effect of Lycopene on Hypoxia/Reoxygenation Injury in Rat Cardiomyocytes and Its Mechanism

Objective：To investigate the protective effects of Lycopene on neonatal rat cardiomyocytes injury induced by hypoxi-a/reoxygenation（H/R）and to explore the mechanism.Methods：The H/R injury model of primary cultured neonatal rat cardiomyocytes was established.The cultured cardiomyocytes were divided into eight groups：normal control group,H/R group,H/R＋Lycopene（1,2,4,8,16,32 μmol/L） group.The activities of aspartate aminotranferase（AST）,creatine kinase（CK）,lactate dehydrogenase（LDH）,su-peroxide dismutase（SOD） and the content of malondialdehyde（MDA） were determined.The numbers of apoptotic cardiomyocyte was determined by MTT,The expression of TLR-4 protein and NF-κB were detected by Western blot.Results：Lycopene groups（16,8,4,2 μmol/L） decreased the activities of AST,CK,LDH and the production of MDA,and significantly increased the activity of SOD.Com-pared with H/R group,Cardiomyocyte apoptosis in Lycopene group was significantly lower than that in H/R group but it was higher than that in the normal control group（P 0.05）.The expression of TLR-4 protein and NF-κB protein in Lycopene group was significantly 1ower than that in the H/R group but it was higher than that in the normal control group.（P 0.05）.Conclusion：Lycopene has the effect of anti-hypoxia / reoxygenation injury and protection of myocardial cells,which may be achieved by inhibiting the pathway to TRL4.