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EDU免疫荧光法检测整合素连接激酶(ILK)高表达对新生大鼠心肌细胞DNA合成的影响
引用本文:王丙剑,白剑,孙静娴,张娜,徐标.EDU免疫荧光法检测整合素连接激酶(ILK)高表达对新生大鼠心肌细胞DNA合成的影响[J].生物磁学,2011(10):1858-1860.
作者姓名:王丙剑  白剑  孙静娴  张娜  徐标
作者单位:[1]南京医科大学附属鼓楼临床医学院心脏科,江苏南京210008 [2]南京大学医学院附属鼓楼医院心脏科,江苏南京210008
基金项目:国家自然科学基金项目(81070195)
摘    要:目的:通过EDU免疫荧光法观察整合素连接激酶(Integrin-Linked Kinase,mK)在新生大鼠心肌细胞内高表达后对心肌细胞DNA合成的影响。方法:取新生(1-3天内)大鼠原代心肌细胞,培养72小时后随机分为正常对照组、ILK转染组。对照组转染重组腺病毒载体(adeno-GFP),ILK纽转染重组腺病毒载体+ILK基因(adeno-ILK)。转然成功后48小时将两组心肌细胞分别通过5-乙基-2-脱氧尿嘧啶核苷(EDU)免疫荧光法测定心肌细胞DNA合成。结果:ILK转染组心肌细胞内DNA合成较对照组明显增加(P〈O.05)。结论:ILK高袁达具有促进新生大鼠心肌细胞的DNA合成的能力。

关 键 词:整合素连接激酶  心肌细胞  增殖  DNA合成  EDU

Detection of Over-Expressed Integrin-Linked Kinase in DNA Synthesis of Cardiomyocytes in Newborn Rats by EDU Immunofluorescence Assay
WANG Bing-jian,BAI Jian,SUN Jing-xian,ZHANG Na,XU Biao.Detection of Over-Expressed Integrin-Linked Kinase in DNA Synthesis of Cardiomyocytes in Newborn Rats by EDU Immunofluorescence Assay[J].Biomagnetism,2011(10):1858-1860.
Authors:WANG Bing-jian  BAI Jian  SUN Jing-xian  ZHANG Na  XU Biao
Institution:1 Department of Cardiology, Drum Tower Clinical Medical College, Nanjing Medical Universi 210008, Jiangsu, China; 2 Department of Cardiology ,Nanjing Drum Tower Hospital, Nanjing University Medical School, 210008,Jiangsu, China)
Abstract:To investigate the effects of over-expressed integrin-linked kinase on rat neonatal DNA synthesis of cardiomyoeytes by EDU immunofluorescence assay. Methods: Primary neonatal rat (1-3 days old) cardiomyocytes were cultured in vitro and randomly divided into two groups 72 h after isolation: infected by either adeno-GFP (control group) or adeno-GFP containing adeno-lLK (ILK group). 48h after infection, DNA synthesis of cardiomyocytes in two groups were determined by 5-ethynyl-2-deoxyuridine(EdU) immunofluorescence assay. Results: Compared with the control groups, DNA synthesis of cardiomyocytes increased dramatically in ILK groups (P 〈0.05). Conclusion: The over-expression of ILK could promote Rat Neonatal DNA synthesis of cardiomyocytes.
Keywords:Integrin-linked kinase  Cardiomyocyte  proliferation  DNA synthesis  EDU
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