不同RF型类风湿性关节炎患者Th17相关细胞因子表达研究

目的：探讨类风湿因子阳性与阴性类风湿关节炎（rheumatoid arthritis,RA）患者外周血中辅助T细胞（Th17）及相关细胞因子白介素17（interleukin,IL-17）,白介素6（interleukin,IL-6）表达的差异。方法：收集RA患者51例,根据RF测定分为RF＋、RF-组,健康查体者（对照组）20例,采用流式细胞术检测受检者外周血单个核细胞（PBMC）的Th17细胞的百分率;以酶联免疫吸附法（ELISA）检测受检者血浆中IL-17,IL-6的水平。结果：RA患者CD4＋IL-17＋T细胞,IL-17、IL-6水平均高于对照组,RF因子阳性与阴性RA患者之间CD4＋IL-17＋T细胞,IL-17、IL-6表达水平均存在差异有统计学意义。结论：在RA中不同RF型免疫反应和炎症表达的不同,可能与Th17及相关细胞因子表达差异有关。

Expression of Th17 Type Cytokines in Rheumatoid Arthritis Patients with RF~＋ and RF~-

Objective：To investigate the different expression of Th17 type cytokines in the peripheral blood of rheumatoid arthri-tis patients with rheumatoid factor positive（RF＋） and rheumatoid factor negative（RF-）.Methods：51 RA patients including RF＋ and RF-and 20 healthy subjects were studied.The percentage of Th17 cell in single PBMC was determined by flow cytometry and the levels of IL-17 and IL-6 in plasma were detected by Enzyme-linked immunosorbent assay（ELISA） in all subjects.Results：Th17 cell and the con-centrations of IL-6 and IL-17 were significantly higher in RA patients than in healthy controls.Furthermore,Th17 and the concentra-tions of IL-6 and IL-17 in RF＋ cases were higher than that in RF-cases.Conclusions：Different immune reaction and inflammation ex-pression in RA patients with or without RF may relate with the differences of the expression of Th17 type cytokine.