藏红花提取液对去卵巢大鼠骨密度及骨代谢生化指标的影响

目的：研究中药藏红花提取液对去卵巢大鼠股骨骨密度及血清骨代谢生化指标的影响。方法：选用48只4月龄SD雌性大鼠,随机分为6组：假手术组、模型组、戊酸雌二醇组、藏红花低、中、高剂量组。术后4周各组分别给予相应制剂灌胃,术后12周处死,分别测定股骨骨密度、子宫指数、雌二醇、血钙、血磷、碱性磷酸酶。结果：与模型组相比,藏红花各剂量组股骨骨密度明显升高（p〈0.01）,雌二醇测定值升高（p〈0.01）,碱性磷酸酶显著降低（p〈0.01）,血钙及血磷无统计学差异（p〉0.05）;与戊酸雌二醇组比较,藏红花各剂量组子宫指数显著降低（p〈0.01）。结论：藏红花提取液有助于抑制去卵巢大鼠骨量的丢失,改善骨代谢,对骨质疏松症具有防治作用。

Effects of Crocus Sativus Extract on Bone Mineral Density and Biochemistry Markers of Bone Metabolism in Ovariectomized Rats

Objective：To investigate the effects of Crocus sativus extraction on bone mineral density（BMD） and biochemistry markers of bone metabolism in ovariectomized rats.Methods：Forty-eight female Sprague-dawley rats,four months old,were randomly divided into six groups：the sham-operating group（Sham）,the ovarictomized group（OVX）,estradiol valerate treated group（E2）,low-dose of Crocus sativus treated group（L-Crocus）,medium-dose of Crocus sativus treated group（M-Crocus） and high-dose of Crocus sativus treated group（H-Crocus）.Four weeks after surgery,the rats of Sham group and OVX group were intra-gastric administrated with distilled water,while the rats of the E2 group received estradiol valerate（0.8mg/kg.d） and rats of other three groups received different dosage of Crocus sativus extract（25,50,100mg/kg.d） by gavage for 8 weeks.Then all the rats were executed and their right femoral bones and blood sera were collected.The BMD,index of uterus,serum estrogen levels and serum biochemical markers of bone metabolism were determined.Results：The BMD and serum estrogen levels in the rats of three Crocus sativus extract treated groups were significantly higher than that in OVX group（p0.01）,while the serum alkaline phosphatase（ALP） level in the former were obviously lower than that in the latter（p0.01）.Compared with the E2 group,the index of uterus decreased significantly in three Crocus sativus extract treated groups（p0.01）.Conclusion：Crocus sativus showed the potential effects on prevention of osteoporosis in ovariectomized rats by inhibiting the bone mineral loss and improving the bone metabolism.