蚕丝蛋白和明胶复合组织工程材料与肝细胞相容性的实验研究

目的：研究蚕丝蛋白-明胶三维材料支架对人永生化肝细胞系QZG贴附及增殖的影响。方法：采用四氮唑盐比色法（MTT）、细胞计数法检测QZG细胞在纯蚕丝生物材料上与在蚕丝蛋白-明胶复合材料上的增殖情况,用扫描电镜观察QZG细胞在两种三维生物材料上的贴附与增殖情况。结果：QZG细胞可以在蚕丝蛋白生物材料贴附及增殖,在引入明胶的蚕丝蛋白材料上细胞贴附更紧密,增殖更明显。结论：蚕丝蛋白与明胶复合材料支架具有良好的细胞贴附性能,通过改进在肝组织工程应用方面将具有一定应用前景。

Experimental Study on Silk Protein-Three-dimensional Gelatin Scaffold and Compatibility of Liver Cells

Objective： To study the silk fibroin material＇s effect on proliferation of human normal hepatic cell line QZG,and to testify the cytocompatibility when blending of silk fibroin and gelatin into 3D scaffold.Methods：The activity of cell proliferation and relative growth rate（RGR） were assessed by MTT colorimetric assay and viable cell counting,The effect of silk protein materials on growth of cells was observed by scanning electron microscopy（SEM）.Results： Human normal hepatic cell line QZG were cultivated in vitro directly on pure silk protein materials and silk fibroin/ gelatin materials.Cells solidly adhered to the materials.The activity of cell proliferation on silk fibroin/ gelatin materials was significantly more than that in the group of pure silk fibroin materials.The result of viable cell counting was compatible to MTT colorimetric assay.Conclusion：The silk fibroin materials together with gelatin has a good cytocompatibility with human normal hepatic cell line QZG.It will be useful in liver tissue engineering.