神经元特异性烯醇酶和癌胚抗原胶体金免疫层析联合检测

目的：应用双抗夹心胶体金免疫层析方法,实现对神经元特异性烯醇化酶（NSE）和癌胚抗原（CEA）两种肺癌肿瘤标志物的快速联合检测。方法：采用柠檬酸三钠还原法制备20nm胶体金颗粒,并分别对鼠抗NSE、CEA单克隆抗体进行标记,分别与之相配对的另一种单克隆抗体被喷在硝酸纤维素膜（NC膜）上,制成免疫层析检测试条。溶液中的抗原NSE、CEA与金标记抗体结合后沿着硝酸纤维素膜移动,与膜上固定的抗体结合形成肉眼可见的红色线条。结果：该试纸条只与NSE、CEA有特异性反应,与CA125、CYFRA21-1、TPA等肺癌标志物无交叉反应。标准样品中两种抗原的检测灵敏度分别可达到5ng/mL和3ng/mL。结论：胶体金免疫层析技术检测NSE、CEA特异性强、灵敏度高、简便快速,不需特殊仪器设备,有广泛应用价值。

Joint Detection of Neuron-specific Enolase and Carcinoembryonic Antigen with Gold Immunochromatography Assay（GICA）

Objective：To realize the rapid joint detection for Neuron-specific enolase and carcinoembryonic antigen by double antibodies sandwich gold immunochromatography assay.Methods：Colloidal gold with diameter 20nm was prepared through reducing chloroauric acid with trisodium citrate.Gold nanoparticles were coupled with monoclonal anti-NSE/CEA.Another corresponding monoclonal antibody to the two antigens was fixed on the NC membrane to prepare GICA test strips.The gold-anti-NSE/CEA conjugate reacts with NSE/CEA in solution and migrates on the nitrocellulose membrane on which anti-NSE/CEA was immobilized,and producd the visual red lines.Results：This kind of test strips could react with NSE and CEA specificly,and there were no cross-reactions with other lung cancer markers,such as CA125,TPA,CYFRA21-1.The sensitivity of NSE was 5ng/mL and CEA was 3ng/mL by detecting the standard samples.Conclusion：GICA is a sensitive,specific,simple and rapid assay for detecting NSE and CEA.It need not any special devices and has significant practical value.