| PCR防污染技术的研究进展 |
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| 引用本文： | 王璐茜,邹秉杰,周国华.PCR防污染技术的研究进展[J].生物磁学,2011(14):2797-2800. |
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| 作者姓名： | 王璐茜 邹秉杰 周国华 |
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| 作者单位： | [1]中国药科大学生命科学与技术学院,江苏南京210009 [2]华东医学生物技术研究所,江苏南京210002 |
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| 基金项目： | 江苏省自然科学基金（No.BK2010111）; 国家自然科学基金项目（No.21005088） |
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| 摘 要： | 聚合酶链式反应（PCR）是一种高灵敏核酸扩增技术,广泛应用于核酸检测中。但在实际应用过程中,扩增产物及其他核酸片段的污染会导致假阳性的结果,制约了PCR在临床检测中的应用。为了解决这一问题,建立了许多PCR防污染的方法,除了早期建立的并已得到广泛应用的物理隔绝法、光照法及水解法外,近年来还发展了酶消化法、化学修饰法及DEAE纤维素法。本文对PCR防污染技术的原理、应用及进展进行了综述。
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| 关 键 词： | PCR 防污染 UNG DNaseI 限制性内切酶 |
Research Progress of PCR Decontamination Methods |
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| WANG Lu-qian,ZOU Bing-jie,ZHOU Guo-hua.Research Progress of PCR Decontamination Methods[J].Biomagnetism,2011(14):2797-2800. |
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| Authors: | WANG Lu-qian ZOU Bing-jie ZHOU Guo-hua |
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| Institution: | 1 College of Life Science and Technology,China Pharmaceutical University,Nanning 210009,China; 2 Huadong Research Institute for Medicine and Biotechnics,Nanning 210002,China) |
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| Abstract: | Polymerase chain reaction（PCR） is a highly sensitive nucleic acid amplification technique,which has been widely used in nucleic acid detection.However,the contamination of amplicons or other nucleic acid fragments,which usually leads to false-positive results,limits it＇s clinical applications.To solve this problem,many approaches were developed to eliminate PCR contami-nation.Besides early established methods based on physical separation,irradiation and hydrolysis,methods by using enzyme digestion,chemical modification and DEAE cellulose are developed recently.In this paper,the principles,applications and advances of PCR decon-tamination methods are reviewed. |
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| Keywords: | PCR Decontamination UNG DNase I Restriction endonuclease |
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