| RP-HPLC法测定酸枣仁汤中甘草苷的含量 |
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| 引用本文： | 齐凤琴,李延利,张冰,张博,郭冷秋,黄莉莉.RP-HPLC法测定酸枣仁汤中甘草苷的含量[J].生物磁学,2011(13):2543-2545. |
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| 作者姓名： | 齐凤琴 李延利 张冰 张博 郭冷秋 黄莉莉 |
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| 作者单位： | 黑龙江中医药大学,黑龙江哈尔滨150040 |
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| 基金项目： | 2008年教育部博士点基金（200802280001） |
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| 摘 要： | 目的：建立测定酸枣仁汤中甘草苷含量的反相高效液相色谱分析方法。方法：色谱柱：WatersC18（250nm×4．6mm，5wm）；流动相：乙腈一0．1％磷酸水溶液，梯度洗脱；流速：1．0ml／min，检测波长：276nm。结果：芒果苷在0．093μg·0．744μg范围内与峰面积线性关系良好（r==0．9999），平均加样回收率为98．04％，RSD为0．41％。结论：本方法简便、准确、可靠、重复性好，可作为酸枣仁汤中甘草苷的含量测定方法。
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| 关 键 词： | 酸枣仁汤 芒果苷 RP—HPLC |
Determination of Liquiritin in Suanzaoren Decoction by RP-HPLC |
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| Institution: | QI Feng-qin, LI Ting-li ,ZHANG Bing, ZHANG Bo (Heilongjiang University of Traditional Chinese Medicine, Haerbin 150040, China) |
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| Abstract: | Objective： To establish a HPLC method for the determination of Liquiritin in Suanzaoren Decoction. Methods：The determination was performed on WatersC 18 column （250nm ×4.6mm,5μm）with a grient mobile phase composed of acetionitrile and 0.1% phosphoric acid. The detection wavelength was 276nm,and the flow rate was lml/min.Results：The Mangiferin showed good linearity （r=0.9999）in the range of 0.093μg-0.744μg, the average recovery was 98.04% with RSD 0.41%. Conclusions：The method is simple, accurate, reliable and is suitable for Determination of Liquiritin in Suanzaoren Decoction. |
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| Keywords: | Suanzaoren Decoction Liquiritin RP-HPLC |
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