23例1型糖尿病初发患儿免疫学改变与胰岛β细胞功能的关系

目的：观察1型糖尿病（T1DM）初发患儿细胞免疫与胰岛β细胞功能之间的关系。方法：T1DM组23例初发患儿均测定淋巴细胞亚群（T细胞、B细胞及NK细胞）、HbA1c、INS、C-P,正常对照组20例,测定淋巴细胞亚群。结果：T1DM组CD4、CD4/CD8较正常对照组升高（P〈0.05）;CD8、CD3-CD56＋较对照组降低（P〈0.05）;CD4/CD8比值与HbA1c呈正相关（r=0.9451,P〈0.01）,而与INS呈负相关（r=-0.1020,P〈0.01）,与C-P呈负相关（r=-0.6174,P〈0.01）;CD3-CD56＋与HbA1c呈正相关（r=0.1320,P〈0.01）,而与INS呈正相关（r=-0.0846,P〈0.01）,与C-P呈负相关（r=-0.3224,P〈0.01）。结论：T1DM初发患者CD4、CD4/CD8明显增高,CD8、CD3-CD56＋明显降低,提示细胞免疫功能改变与胰岛β细胞功能损伤密切相关。

Relationship between Immunological Change and Function of Islet Beta Cell in 23 Children with Type 1 Diabetes Newly Diagnosed

Objective：To investigate the relationship between cellular immunity and function of islet beta cell in children with type 1 diabetes Newly Diagnosed.Methods：Lymphocyte subsets（T cells,B cells,NK cells）,HbA1c,INS and C-P were detected in diabete group（23 children） with type 1 diabetes newly diagnosed.Lymphocyte subsets were measured in normal control group（20 children）.Results：The CD4 and the CD4/CD8 ratio in diabetes group was higher than that in normal control group（P0.05）.The percentage of CD8 and CD3-CD56＋ decreased compared with that of controls（P0.05）.Both the CD4/CD8 ratio and CD3-CD56＋ were positively correlated with HbA1c.The CD4/CD8 ratio was negatively correlated with INS or C-P.CD3-CD56＋ was positively correlated with INS,but which was negatively correlated with C-P.Conclusions：The percentage of CD4 and the CD4/CD8 ratio in children with type 1 diabetes Newly Diagnosed incresed significantly,while the percentage of CD8 and CD3-CD56＋ decreased significantly,which suggested that the change of cellular immunity was closely related with functional damages of islet beta cell.