| 云芝糖肽在ROS、p38信号通路中对巨噬细胞Raw264．7的免疫调节作用 |
| |
| 引用本文： | 董莎莎,杨晓彤,冯慧琴,杨明俊,杨庆尧.云芝糖肽在ROS、p38信号通路中对巨噬细胞Raw264．7的免疫调节作用[J].生物磁学,2011(15):2848-2850. |
| |
| 作者姓名： | 董莎莎 杨晓彤 冯慧琴 杨明俊 杨庆尧 |
| |
| 作者单位： | [1]上海师范大学微生物与免疫学研究所,上海200234 [2]上海芝草生物技术有限公司,上海200234 |
| |
| 摘 要： | 目的：探究云芝糖hk（PSP）对静息和脂多糖（LPS）过度刺激两种不同状态的巨噬细胞Raw264．7产生活性氧簇（ROS）TLp38、p-p38的影响及其相关机制。方法：采用流式细胞术方法检测ROS的表达；Westernblot方法检测MAPK信号通路中p38磷酸化及非磷酸化的相对表达量。结果：100g／mlPSP下调1g／ml LPS单独作用于巨噬细胞时ROS及p-p38的表达量；100g／mlPSP上调正常巨噬细胞p-p38的表达量；p38表达量基本不变。结论：PSP通过ROS，p38两条通路参与巨噬细胞的免疫调节作用。
|
| 关 键 词： | 云芝糖肽（PSP） 脂多糖（LPS） 巨噬细胞 炎症 ROS p38 |
Immunomodulatory Effects of Coriolus Versicolor Polysaccharopeptide on Macrophages Raw264.7 in ROS and p38 Signaling Pathways |
| |
| DONG Sha-sha,YANG Xiao-tong,FENG Hui-qin,YANG Ming-jun,YANG Qing-yao.Immunomodulatory Effects of Coriolus Versicolor Polysaccharopeptide on Macrophages Raw264.7 in ROS and p38 Signaling Pathways[J].Biomagnetism,2011(15):2848-2850. |
| |
| Authors: | DONG Sha-sha YANG Xiao-tong FENG Hui-qin YANG Ming-jun YANG Qing-yao |
| |
| Institution: | 1 Institutes of Microbiology and Immunology, Shanghai Normal University, SHANGHAI 200234, China; 2 Shanghai Zhi-Herb Biotectmology Co.,Ltd.,SHANGHAI 200234,China) |
| |
| Abstract: | Objective： To investigate the effects and mechanisms of Coriolus versicolor polysaccharopepide （PSP） on the production of Reactive oxygen species （ROS）, p38 and phosphor-p38 on normal and lipopolysaccharides （LPS） stimulated murine macrophage. Methods： ROS was measured by Flow cytometry; p38and phosphor-p38 were detected by Western blot. Results： While stimulated by LPS （1 g/ml）, there was less ROS and phosphor-p38 in cells pretreated with PSP （100g/ml） ; 100g/ml PSP along could increase the expression of phosphor-p38; The amount of p38 were unaffected by either LPS or PSP with LPS. Conclusion： PSP could regulate mouse macrophage immune reactions in ROS and p38 signaling pathways. |
| |
| Keywords: | PSP LPS Macrophages Inflammation ROS p38 |
| 本文献已被 维普 等数据库收录！ |
