| EGCG经AKT1-Mdm-2-p53途径抑制卵巢癌HO-8910细胞的增殖 |
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| 引用本文： | 李维,罗瞳,罗招阳.EGCG经AKT1-Mdm-2-p53途径抑制卵巢癌HO-8910细胞的增殖[J].生物磁学,2011(15):2840-2843. |
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| 作者姓名： | 李维 罗瞳 罗招阳 |
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| 作者单位： | [1]南华大学医学院肿瘤研究所,湖南衡阳421001 [2]南华大学医学院病理学教研室,湖南衡阳421001 [3]湖南浏阳市人民医院妇科,湖南浏阳410300 [4]湖南浏阳市人民医院烧伤科,湖南浏阳410300 |
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| 摘 要： | 目的：初步探讨EGCG对卵巢癌HO-8910细胞增殖的抑制作用及其机制。方法：通过绘制细胞生长曲线、平皿克隆和软琼脂集落形成实验观察EGCG对HO-8910细胞增殖的抑制作用；Westem—blotting检测AKT1、Mdm-2与p53蛋白的表达。结果：（1）细胞生长曲线、平皿克隆和软琼脂集落形成实验结果显示，EGCG可有效抑制HO-8910细胞的增殖（n=3，P〈0．05）。（2）Western—blotting检测结果显示，EGCG处理后AKT1与Mdm-2蛋白表达均降低，而p53蛋白表达升高（P〈0．05）。结论：EGCG通过抑制HO-8910细胞中AKT1与Mdm-2蛋白表达，促使p53蛋白表达而发挥其对细胞增殖的抑制作用。
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| 关 键 词： | EGCG 卵巢癌 HO-8910细胞 AKT1蛋白 Mdm-2蛋白 p53蛋白 |
Epigallocatechin-3-gallate Inhibits Proliferation of Ovarian Cancer HO-8910 Cells by AKT 1-Mdm-2-p53 Pathway |
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| LI Wei,LUO Tong,LUO Zhao-yang.Epigallocatechin-3-gallate Inhibits Proliferation of Ovarian Cancer HO-8910 Cells by AKT 1-Mdm-2-p53 Pathway[J].Biomagnetism,2011(15):2840-2843. |
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| Authors: | LI Wei LUO Tong LUO Zhao-yang |
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| Institution: | 1. Cancer Research Institute oMedical College, 2 Department of Pathology o EMedical College, University of Southern China, Hengyang, Hunan 421001, China; 3 Department oGynecology; 4.Department oBurn, People＇shospital of Liuyang City, Liuyang, Hunan 410300) |
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| Abstract: | Objective： To investigate mechanism of Epigallocatechin-3-gallate （EGCG） inhibited the proliferation of ovarian cancer HO-8910 cells. Methods： EGCG inhibited HO-8910 cellular proliferation was observed by the drawing cellular growth curve, plate colony formation and soft agar colony formation. The expressions of AKT1, Mdm-2 and p53 proteins were detected by Western-blotting. Results： 1. The results of cellular growth curve, plate colony formation and soft agar colony formation showed that EGCG active depressed the proliferation of HO-8910 cells with treatment time extended （n=3, P 〈 0.05）. 2. The western-blotting results exhibited that the expressions of AKT1 and Mdm-2 proteins in HO-8910 cells obviously decreased, but the expression of p53 protein increased after EGCG dealing with it （P 〈 0.05）. Conclusion： EGCG depressed the expressions of AKT1, Mdm-2 proteins in HO-8910 cells and increased the expression of p53 protein to inhibit the cellular proliferation. |
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| Keywords: | Epigallocatechin-3-gallate Ovarian cancer HO-8910 cell AKT1 protein Mdm-2 protein p53 protein |
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