慢性充血性心力衰竭大鼠模型的建立

目的：探讨建立慢性充血性心力衰竭大鼠模型的方法。方法：采用腹主动脉缩窄法制备CHF大鼠模型。于右肾动脉分支处上方缩窄腹主动脉至0.6mm,同时设立假手术组及正常对照组。12周后行一般情况观察,超声心动图及血液动力学检测心脏结构和功能、计算心脏肥厚指数,HE及Masson染色检测心肌病理改变,综合评价CHF大鼠心功能。结果：与假手术及正常对照组比较,CHF模型组术后12周均出现进食减少、精神萎靡、少动、被毛无光;IVS、LVPW、LVM、LVEDD、LVESD、SV显著增加,EF、FS显著降低;HR显著增加;VSP、＋dP/dtmax显著降低,T-dP/dtmax显著延长;LVEDP显著升高,-dP/dtmax绝对值显著降低;心脏湿重及心脏肥厚指数均增加;病理结果表明CHF模型组心肌细胞肥大、排列紊乱、心肌胶原纤维增多。结论：应用腹主动脉缩窄术可成功制备CHF大鼠模型。

Establishment of Rat Models with Chronic Congestive Heart Failure

Objective：To establish rat models with chronic congestive heart failure.Methods：The CHF rats were prepared by abdominal aorta constriction to 0.6mm.After 12 weeks,the observation of general condition,echocardiagraphy,hemodynamics parameters,pathological HE staining and Masson staining were all performed to evaluate the cardiac function of CHF rats.Results：The general condition of CHF rats were poor.Echocardiagraphy parameters showed IVS,LVPW,LVM,LVEDD,LVESD,SV increased,and EF,FS decreased.Hemodynamics parameters showed HR,LVEDP,T-dP/dtmax increased,and LVSP,dP/dtmax decreased.The heart wet weight and heart mass index of CHF rats were both increased.Pathological results showed there were hypertrophic and disordered cardiomyocyte and proliferation of fibrous tissue.Conclusion：CHF rats were established by abdominal aorta constriction.