食管鳞癌与腺癌差异基因表达的对比分析

目的：研究食管鳞癌（esophageal squamous cell carcinoma，ESCC）与食管腺癌（esophageal adenocarcinoma，EAC）的基因差异表达，探讨ESCC与EAC发生发展的基因学基础。方法：选取8例ESCC和8例EAC组织抽提mRNA，应用cDNA芯片技术通过芯片杂交、生物信息学处理，找出两者间差异表达基因。结果：采用BioStarH-40芯片发现差异表达基因541条，差异表达基因占13．8％，其中表达增强309条（显著增强73条），表达降低232条（显著降低61条）。结论：ESCC与EAC基因表达比较，差异有统计学意义，这些差异可能在两类肿瘤不同的生物学行为中起重要作用。

Differences of Gene Expression Profile between Esophageal Squamous Cell Carcinoma and Esophageal Adenocarcinoma

Objective： To determine the diference in the gene expression between human esophageal squamous cell carcinoma （ESCC） and esophageal adenocarcinoma （EAC）. Methods： cDNA chip was used to detect the mRNA of cancer tissue from 8 ESCCs and 8 EACs. After profile bloted and handled by bioinformation,the gene expression of these two kinds of lesions was examined. Results： Using the BioStarH-40 profile, 541 diferent expression of genes was found. The rate of different genes was 13.8%, of which the exprssion of 309 genes increased （73 genes significantly increased） and 232 genes decreased （61 genes significantly decreased ） in ESCC tissue than that in EAC. Conclusions： The gene expression of ESCC and EAC was different, which may contribute to the different biological behavior of these two kinds of esophageal cancer.