| Neurospora crassa phytase功能区基因phy A＇的克隆表达及活性变化 |
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| 引用本文： | 周小玲,;黄天华,;诸葛健.Neurospora crassa phytase功能区基因phy A＇的克隆表达及活性变化[J].生物磁学,2008(11):2018-2020. |
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| 作者姓名： | 周小玲 ;黄天华 ;诸葛健 |
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| 作者单位： | [1]汕头大学医学院,广东汕头515041; [2]江南大学生物工程学院,江苏无锡214036 |
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| 基金项目： | 汕头大学博士后科研启动基金 |
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| 摘 要： | 目的：检测N.crassa phtase功能区基因phy A′表达的Phy A′活性变化。方法：先将N.crassa phy A′克隆到表达载体pPIC9K中,然后将获得的重组质粒pPIC9K-phy′转化到毕赤氏酵母中分泌表达,分离纯化重组酶Phy A′,并测定其酶学性质。结果：与原酶相比,N.crassa Phy A′的最适温度降低、热稳定性降低以及酶活力的降低。结论：N.crassa phtase N端的前12个氨基酸对维持该酶的功能有比较重要的作用。
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| 关 键 词： | 植酸酶 功能区基因 活性 |
Clone,Expression and Activity Change of Neurospora crassa Phytase phy A＇ Motif |
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| Institution: | ZHOU Xiao-ling, Huang Tian-hua, Zhuge Jian (1 Medical Colllege;Shantou University;Guangdong Shantou 515041;China;2 College of Biotechnology;Jiangnan University;Wuxi 214036;China) |
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| Abstract: | Objective：To investigate the enzyme activity of N.crassa phytase phy A＇motif.Methods：N.crassa phtase phy A＇ was cloned into the expression vector pPIC9K.Then,the recombinant plasmid pPIC9K- phy＇ was transformed into Pichia pastoris KM71 to express and purify the recombinant enzyme Phy A＇.And the activity of Phy A＇ was determined.Results：The temperature optimum,thermostability and activity of Phy A＇ decreased compared with N.crassa phrase.Conlusions：The 12 amino acids of N-terminal play an important role in maintaining the activity of N.crassa phytase. |
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| Keywords: | Phytase Motif Activity |
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