Comparison of C18-Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovis in Bovine Milk Samples and Analysis of Samples by PCR |
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| Authors: | Brandon J Cornejo Alfredo Sahagún-Ruiz Francisco Surez-Güemes Charles G Thornton Thomas A Ficht and L Garry Adams |
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| Institution: | Brandon J. Cornejo, Alfredo Sahagún-Ruiz, Francisco Suárez-Güemes, Charles G. Thornton, Thomas A. Ficht, and L. Garry Adams |
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| Abstract: | The purpose of this prospective study was to compare two different milk preparation methods to assay for the presence of Mycobacterium bovis by PCR. Detection by a C18-carboxypropylbetaine (CB-18)-based sample processing method was compared to extraction of DNA from milk with glass beads. Samples from 17 skin test-positive cattle were analyzed. Following CB-18 processing and glass bead extraction, the sensitivity of IS6110-based PCR was 94.1 and 58.8%, respectively (P < 0.025). Because CB-18 processing will permit the proficient use of PCR for diagnosis and surveillance of bovine tuberculosis, it will contribute to the more efficient detection and control of tuberculosis. |
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