Melatonin reduces inflammatory injury through inhibiting NF-kappaB activation in rats with colitis |
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Authors: | Li Jun-Hua Yu Jie-Ping Yu Hong-Gang Xu Xi-Ming Yu Liang-Liang Liu Jin Luo He-Sheng |
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Institution: | Department of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan, China. |
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Abstract: | Proinflammatory mediators are important in the pathogenesis of IBD, which are regulated by activation of NF-kappaB. The aim of this study was to investigate whether melatonin reduces inflammatory injury and inhibits proinflammatory molecule and NF-kappaB in rats with colitis. Rat colitis model was established by TNBS enema. NF-kappaB p65, TNF-alpha, ICAM-1, and IkappaBalpha in colon tissue were examined by immunohistochemistry, EMSA, RT-PCR, and Western blot analysis. Expression of proinflammatory molecule and activation of NF-kappaB were upregulated and IkappaB level decreased in rats with colitis. Melatonin reduces colonic inflammatory injury through downregulating proinflammatory molecule mediated by NF-kappaB inhibition and blockade of IkappaBalpha degradation. |
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