Sixteen novel microsatellite loci developed for the giant panda (Ailuropoda melanoleuca) |
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| Authors: | He-Min Zhang Yang Guo De-Sheng Li Peng-Yan Wang Sheng-Guo Fang |
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| Institution: | (1) China Research and Conservation Center for the Giant Panda, Wenchuan, 623006, P.R. China;(2) College of Life Sciences, Zhejiang University, State Conservation Center for Gene Resources of Endangered Wildlife and the Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Zi Jin Gang Campus, Hangzhou, 310058, P.R. China |
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| Abstract: | Sixteen novel microsatellite DNA loci were developed from the giant panda (Ailuropoda melanoleuca) using a magnetic-bead capture method. A total of 115 alleles were obtained for these markers, ranging from 4 to 12 alleles
per locus (average 7.188). These loci exhibited high levels of polymorphic information content and expected heterozygosity,
0.558–0.855 (average 0.729) and 0.628–0.885 (average 0.778), respectively. Therefore, the allelic polymorphism and heterozygosity
show that the giant pandas raised in China Research and Conservation Center possess abundant genetic variation. In addition,
if the three markers showing null alleles were excluded, the remaining 13 microsatellite loci still presented extremely low
non-exclusion probabilities of parentage (0.002), paternity (0.000) and identity (0.000). As a result, this new suit of microsatellite
markers would be a very informative tool for the genetic and conservation studies of giant pandas. |
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| Keywords: | Genetic diversity Allele Polymorphism Exclusion probability |
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