Development and characterization of microsatellite loci in the endangered Tricyrtis ishiiana (Convallariaceae), a local endemic plant in Japan |
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| Authors: | Hiroaki Setoguchi Yuki Mitsui Hajime Ikeda Naofumi Nomura Atsushi Tamura |
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| Institution: | (1) Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan;(2) Kanagawa Prefecture Natural Environment Conservation Center, Nanasawa 657, Atsugi, 243-0121, Japan |
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| Abstract: | Nine microsatellite loci were isolated and characterized for the endangered and tertiary relict perennial herb, Tricyrtis ishiiana. The number of alleles ranged from 2 to 33. The expected (H
E) and observed (H
O) heterozygosities were 0.207–0.944 and 0.215–0.813, respectively, from 96 individuals on one population. Five loci exhibited
significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish
conservation strategy. |
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| Keywords: | Conservation genetics Convallariaceae Heterozygosity Microsatellite Tricyrtis ishiiana |
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