Isolation and characterization of 11 microsatellite loci from Carex macrocephala (Cyperaceae) |
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| Authors: | Matthew G King Eric H Roalson |
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| Institution: | (1) Department of Botany, University of British Columbia, Vancouver, BC, V6T1Z4, Canada;(2) School of Biological Sciences, Washington State University, Pullman, WA 99164, USA;(3) Center for Integrated Biotechnology, Washington State University, Pullman, WA 99164, USA |
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| Abstract: | Carex macrocephala (Cyperaceae) is a species of great conservation concern along the northwest coast of North America. Using a biotinylated-streptavidin
bead protocol, we successfully isolated and characterized 11 microsatellite loci from a C. macrocephala genomic library. Although these loci are polymorphic, the scored populations exhibit a significant deficiency in heterozygosity.
Carex macrocephala is a self-pollinating rhizomatously spreading sedge, consequently this may contribute to the observed patterns. |
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| Keywords: | Sedge Clonal population genetics Coastal organism Genetic diversity |
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