Isolation and characterization of 12 novel microsatellite loci for the red panda (Ailurus fulgens) |
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Authors: | Hua Wu Xiang-Jiang Zhan Yu Guo Zejun Zhang Lifeng Zhu Li Yan Ming Li Fu-Wen Wei |
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Institution: | (1) Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, People’s Republic of China |
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Abstract: | The information on dispersal patterns and mating systems of red pandas is quite important for the understanding of the genetic
diversity and divergence of this species. And microsatellite marker is an ideal tool to analyze dispersal patterns and mating
systems. Thus, we describe in this paper the isolation and characterization of 12 microsatellite loci in the red panda from
genomic DNA-enriched libraries. These loci were highly polymorphic with numbers of alleles per locus in 24 individuals ranging
from 2 to 14, observed heterozygosity from 0.143 to 0.864 and expected heterozygosity from 0.297 to 0.872. All loci except
for RP6 locus followed Hardy–Weinberg expectations. No significant linkage association was found among all these loci. The
12 novel polymorphic microsatellite loci will be of use in studying dispersal patterns and mating systems of red pandas. |
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Keywords: | Red panda (Ailurus fulgens) Microsatellites Heterozygosity Dispersal patterns Mating systems |
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