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Characterization of 14 novel microsatellite loci in the endangered <Emphasis Type="Italic">Liriodendron chinense</Emphasis> (Magnoliaceae) and cross-species amplification in closely related taxa
Authors:Xiaohong Yao  Jinju Zhang  Qigang Ye  Hongwen Huang
Institution:(1) Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan, Hubei, 430074, China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China
Abstract:Fourteen polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Liriodendron chinense (Magnoliaceae). The average allele number of these microsatellites was 5.2 per locus, ranging from three to eight. The observed and expected heterozygosity at population level were 0.07–1.00 and 0.10–0.83, respectively. These sets of microsatellites will be useful for studies of population genetic structure of Lchinense and L. tulipifera as well as to estimate fine-scale gene flow rates.
Keywords:Liriodendron           chinense                      Liriodendron tulipifera            Magnoliaceae  Microsatellite  Endangered species  Conservation
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