Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of obscure puffer (Takifugu obscurus) and cross-species amplification |
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| Authors: | Hongyu Ma Songlin Chen Xiaolin Liao Tianjun Xu Jiachun Ge |
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| Institution: | (1) Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao, 266071, China;(2) College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China;(3) Freshwater Fisheries Research Institute, Nanjing, 210017, China |
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| Abstract: | Obscure puffer (Takifugu obscurus) is an anadromous fish species in China. Here, we reported 10 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of T. obscurus. The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from four to 10, from 0.57
to 0.86 and from 0.68 to 0.90, respectively. Three loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni
correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification of these
microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be useful
for investigating genetic population structure and construction of genetic linkage map in T. obscurus. |
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| Keywords: | Obscure puffer Takifugu obscurus Microsatellite Enriched genomic library |
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