Genetic diversities of the giant panda (Ailuropoda melanoleuca) in Wanglang and Baoxing Nature Reserves |
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Authors: | Wei He Ling Lin Fujun Shen Wenping Zhang Zhihe Zhang Emily King Bisong Yue |
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Institution: | (1) Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Science, Sichuan University, Chengdu, Sichuan, 610064, P.R. China;(2) Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, 610081, P.R. China;(3) College of Science, Honghe University, Mengzi, Yunnan, 661100, China |
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Abstract: | Genetic variations in the giant panda populations in Wanglang and Baoxing Nature Reserves were evaluated in this study. Panda
feces were collected from these two reserves and DNA samples extracted from the feces were genotyped at 13 microsatellite
loci. A total of 130 alleles were identified from the 13 microsatellite loci in 63 giant pandas, including 35 private alleles
in Wanglang, 53 private alleles in Baoxing, and 42 alleles shared between the two populations. The mean observed heterozygosity,
average number of alleles, average number of allelic richness, and average polymorphism information content were 0.488, 6.2,
3.302, and 0.612, respectively for the Wanglang population and 0.553, 7.6, 4.050, and 0.747 for the Baoxing population. A
moderate degree of genetic differentiation (F
st = 0.26) and no gene flow were found between these two populations.
W. He and L. Lin contributed equally to this work. |
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Keywords: | Giant panda Microsatellite Genetic diversity Gene flow Assignment tests |
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