Development and characterization of microsatellite markers for an endangered shrub, <Emphasis Type="Italic">Ammopiptanthus mongolicus</Emphasis> (Leguminosae) and cross-species amplification in <Emphasis Type="Italic">Ammopiptanthus nanus</Emphasis> |
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| Authors: | Guo-Qing Chen Hong-Wen Huang Ming Kang Xue-Jun Ge |
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| Institution: | (1) South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China;(2) State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China;(3) Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan, 430074, China;(4) Graduate School of the Chinese Academy of Sciences, Beijing, China |
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| Abstract: | We developed 11 polymorphic microsatellite markers for an endangered shrub Ammopiptanthus mongolicus using the Fast Isolation by AFLP of Sequences Containing repeats protocol. Polymorphism of each locus was assessed in 24
individuals from five natural populations of A. mongolicus. The average allele number of these markers was 4.2, ranging from 2 to 7. The observed (H
O) and expected (H
E) heterozygosities were 0.044–0.956 and 0.044–0.827, respectively. Further assessment in the congeneric species A. nanus revealed that nine of eleven loci were successfully amplified, but only two loci showed polymorphism. These markers provide
powerful tools for the conservation genetics studies of A. mongolicus. |
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| Keywords: | Ammopiptanthus mongolicus Endangered species FIASCO Genetic diversity Microsatellite markers |
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