A set of polymorphic nuclear intron markers for conservation genetics and phylogeography of <Emphasis Type="Italic">Euphorbia</Emphasis> species (<Emphasis Type="Italic">Pedilanthus</Emphasis> clade) |
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Authors: | R C Garrick C A Meadows A N Nicolas J D Nason R J Dyer |
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Institution: | (1) Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA;(2) Department of Ecology, Evolution and Organismal Biology, Iowa State University, Ames, IA 50011, USA |
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Abstract: | We developed seven nuclear intron markers for Euphorbia lomelii. New exon-primed intron-crossing (EPIC) oligonucleotides were used for initial amplification and sequencing, then locus-specific
primers and restriction-fragment-length polymorphism genotyping assays were designed. Loci showed no significant deviation
from Hardy–Weinberg and linkage equilibrium, and they cross-amplify in at least three congeneric species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Co-dominant loci Euphorbiaceae Population structure Phylogeny Sonoran Desert |
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