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A set of polymorphic nuclear intron markers for conservation genetics and phylogeography of <Emphasis Type="Italic">Euphorbia</Emphasis> species (<Emphasis Type="Italic">Pedilanthus</Emphasis> clade)
Authors:R C Garrick  C A Meadows  A N Nicolas  J D Nason  R J Dyer
Institution:(1) Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA;(2) Department of Ecology, Evolution and Organismal Biology, Iowa State University, Ames, IA 50011, USA
Abstract:We developed seven nuclear intron markers for Euphorbia lomelii. New exon-primed intron-crossing (EPIC) oligonucleotides were used for initial amplification and sequencing, then locus-specific primers and restriction-fragment-length polymorphism genotyping assays were designed. Loci showed no significant deviation from Hardy–Weinberg and linkage equilibrium, and they cross-amplify in at least three congeneric species. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Keywords:Co-dominant loci  Euphorbiaceae  Population structure  Phylogeny  Sonoran Desert
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